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Your insert has the sequence CTGAATTCTATAATGCCC.....Which pET-41 vector will you use to clone with EcoRI and keep insert in frame? a b C Ncol BamHI EcoRI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC G/AA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met pET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/ GAT CCG/AAT TC Met Asp lle Gly Asp Pro Asn Met Pro pET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CG/A ATT C lle Arg lle Pro Met Ala lle Ser Gly Met

Your insert has the sequence CTGAATTCTATAATGCCC.....Which pET-41 vector will you use to clone with EcoRI and keep insert in frame? a b C Ncol BamHI EcoRI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC G/AA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met pET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/ GAT CCG/AAT TC Met Asp lle Gly Asp Pro Asn Met Pro pET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CG/A ATT C lle Arg lle Pro Met Ala lle Ser Gly Met

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Need help with understanding how to set up, pick and find reading frame for doing a gene insert...

Your insert has the sequence CTGAATTCTATAATGCCC.....Which pET-41 vector will you use to clone with EcoRI and keep insert in frame? a b C Ncol BamHI EcoRI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC G/AA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met pET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/ GAT CCG/AAT TC Met Asp lle Gly Asp Pro Asn Met Pro pET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CG/A ATT C lle Arg lle Pro Met Ala lle Ser Gly Met
Transcribed Image Text:Your insert has the sequence CTGAATTCTATAATGCCC.....Which pET-41 vector will you use to clone with EcoRI and keep insert in frame? a b C Ncol BamHI EcoRI pET-41a(+): ATG...gst...CC/C ATG GGA TAT CGG G/GA TCC G/AA TTC Pro Met Gly Tyr Arg Gly Ser Glu Phe Met pET-41b(+): ATG...gst...CC/C ATG GAT ATC GGG/ GAT CCG/AAT TC Met Asp lle Gly Asp Pro Asn Met Pro pET-41c(+): ATG...gst...CC/C ATG GCG ATA TCG GG/G ATC CG/A ATT C lle Arg lle Pro Met Ala lle Ser Gly Met
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