Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 21.3, Problem 1COMQ
When a dideoxyribonucleotide is incorporated into a growing DNA strand,
a. the strand elongates faster.
b. the strand cannot elongate.
c. the strand becomes more susceptible to DNase I cleavage.
d. none of the above occurs.
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The problem of synthesizing the lagging strand, in the sense that DNA synthesis can only occur in one direction, is manifested inA. creation of primers every so often as more of the template strand isuntwisted.B. formation of shorter DNA strands called Okazaki fragments.C. the need for the enzyme ligase to link short DNA strands together.D. All of the above choices are correct.
One of the mechanisms that leads to the DNA mutations is a process known as DNA polymerase slippage which occurs during DNA replication.
Which of the following statements about DNA polymerase slippage is correct?
A.
Backward slippage in the newly synthesized strand will lead to an insertion mutation.
B.
DNA polymerase slippage can lead to a point or substitution mutation.
C.
Forward slippage in the template strand will lead to an insertion mutation.
D.
All of the above
The function of DNA ligase is to:
a. Catalyze formation of phosphodiester bonds between adjacent nucleotides
b. Catalyze formation of hydrogen bonds between adjacent nucleotides
c. Keep single strands of DNA apart during replication
d. Facilitate base pairing between single stranded molecules in DNA
e. Both a. and d. are correct
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
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- During DNA replication, DNA methylation: please explain answer a. A and C b. B. marks the strand not cut in mismatch repair c. A and B d. A. marks the parental DNA strand e. C. marks the strand cut in mismatch repairarrow_forwardWhich of the following is not a necessary component for DNA synthesis? a. DNA polymerase I b. dNTPs c. Copper ions d. Parental strand of DNA e. All of the above are required for DNA synthesisarrow_forwardDuring DNA replication, which of the following steps occurs first? a. synthesis of the leading strand b. synthesis of the lagging strand c. unwinding of the parental DNA duplex d. sealing of the nicks between short DNA e. synthesis of primersarrow_forward
- Strand invasion is often used to repair which of the following damages to DNA? a. Single-strand breaks b. Double-strand breaks. c. Mismatched bases d. Thymine dimers.arrow_forwardThe elongation of leading strand during DNA synthesis a. progresses away from the replication fork. b. occurs in the 3'-5' direction c. does not require a template strand d. depends on the action of DNA polymerasearrow_forwardDuring DNA replication: A. The two DNA strands separate, each strand then becomes a template for the assembly of a complementary strand. Each new DNA helix has one old strand with one new strand. B. The two DNA strands separate, each strand then becomes a template for the assembly of a similar strand. Each new DNA helix has one old strand with one new strand. C. The two DNA strands separate, each strand then becomes a template for the assembly of a similar strand. Each new DNA helix has two new strands. D. The two DNA strands separate, each strand then becomes a template for the assembly of an identical strand. Each new DNA helix has one old strand with one new strand.arrow_forward
- Which of the following would be most likely to cause a mutation? A. the addition of nucleotides to the 3’ end of the growing strand B. the insertion of a mismatched nucleotide into a DNA strand C. the unwinding of the DNA strand D. the synthesis of short stretches of DNAarrow_forwardWhich of the following enzymes is responsible for ensuring that genetic material at the end of the chromosome is not lost during DNA replication? a. Helicase b. DNA Polymerase c. Telomerase d. DNA Ligase e. Primasearrow_forwarda. unwinds the DNA helix b. stabilizes and stops the two strands from annealing (rebinding with each other) c. recoils the DNA d. cleaves both strands of DNA to relieve tension at supercoils e. places RNA primers at their proper location on the template strands f. acts as starting points for DNA polymerase g. adds DNA nucleotides to form new DNA strands h. forms phosphodiester bonds to join Okazaki fragments Esc 1. single-strand binding protein 2. helicase 3. DNA ligase 4. RNA primer 5. gyrase 6. DNA polymerase 53°F Cloudy 3 Q Search $ F5 FRarrow_forward
- Molecule involved in joining segmented nucleotide strands is... O a. DNA ligase O b. Ribosomes c. RNA polymerase d. DNA polymerase ge F5 F6 F7 F10 Scrol F8 F9arrow_forwardDuring DNA replication, the leading strand is synthesized continuously, while the lagging strand is synthesized as Okazaki fragments. This is because: O A. DNA polymerases can bind to only one strand at a time. O B. Two different DNA polymerases are involved in replication (DNA polymerase I and II). O C. DNA synthesis can take place only in the 5 -> 3 direction. D. The primers are made of RNA. Reset Selection Previous NEXT Save Exit MacBook Pro Q Search or enter website name & %24 %23 4 3 00 < COarrow_forwardWhich of the following statements describe reversal of DNA repair mechanism. A. UV light blocks the translation of mRNA beyond the damaged area B. Reversal of DNA repair mechanism requires a DNA template C. UV light is needed to initiate the formation of pyrimidine dimers D. None of the statement is correctarrow_forward
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