Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
expand_more
expand_more
format_list_bulleted
Videos
Textbook Question
Chapter 21.5, Problem 1COMQ
Which of the following methods use(s) a labeled
a. Site-directed mutagenesis
b. Northern blotting
c. Western blotting
d. Both a and b
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
Which of the following methods is used to detect a specific RNAwithin a mixture of many different RNAs?a. Site-directed mutagenesis c. Western blottingb. Northern blotting d. None of the above
If you are a genetic engineer and you cloned your gene of interest in a plasmid and you
want to know if the protein encoded by the cloned gene is expressed or not, which of
the following methods is the right one to use?
Select one:
a. Northern blot
b. Both Northern and Western blots
c. Agarose gel with polyacrylamide
d. Western blot
e. Protein gel and northern blot
A company that manufactures cleansing products has synthesized two new organic compounds , chemicals A and B, which improve the cleaning power of dishwashing detergents.
To determine the mutagenic capacity of the chemicals , the company tested the effect of the chemicals on Salmonella cells requiring histidine to grow ( His- cells ). The table below shows the results of the test .
A. Name the test used by the company .
B. What does the test detect or estimates ?
C. Which chemical (s) would you identify as containing a mutagen ? Why ?
D. What is the purpose of test 1 ? Why are colonies detected in test 1?
E. Which chemical ( s) would you identify as possible antimutagen ? Why ?
Chapter 21 Solutions
Genetics: Analysis and Principles
Ch. 21.1 - 1. Which of the following may be used as a vector...Ch. 21.1 - The restriction enzymes used in gene-cloning...Ch. 21.1 - 3. Which is the proper order of the following...Ch. 21.1 - 4. The function of reverse transcriptase is...Ch. 21.1 - A collection of recombinant vectors that carry...Ch. 21.2 - Prob. 1COMQCh. 21.2 - Prob. 2COMQCh. 21.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 21.3 - When a dideoxyribonucleotide is incorporated into...Ch. 21.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 21.5 - Which of the following methods use(s) a labeled...Ch. 21.5 - 2. Which of the following methods is used to...Ch. 21.5 - During Western blotting, the primary antibody...Ch. 21.6 - 1. In an EMSA, the binding of a protein to...Ch. 21.6 - The basis for DNase I footprinting is that the...Ch. 21 - Discuss three important advances that have...Ch. 21 - Prob. 2CONQCh. 21 - Write a double-stranded DNA sequence that is 20...Ch. 21 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 21 - 5. Draw the structural feature of a...Ch. 21 - Prob. 1EQCh. 21 - Prob. 2EQCh. 21 - Describe the important features of cloning...Ch. 21 - 4. How does gene cloning produce many copies of a...Ch. 21 - Prob. 5EQCh. 21 - Prob. 6EQCh. 21 - Prob. 7EQCh. 21 - Prob. 8EQCh. 21 - Prob. 9EQCh. 21 - Starting with a sample of RNA that contains the...Ch. 21 - 11. What type of probe is used for real-time PCR?...Ch. 21 - 12. What phase of PCR (exponential, linear, or...Ch. 21 - 13. DNA sequencing can help us to identify...Ch. 21 - A sample of DNA was subjected to automated DNA...Ch. 21 - Prob. 15EQCh. 21 - Prob. 16EQCh. 21 - Prob. 17EQCh. 21 - Prob. 18EQCh. 21 - Prob. 19EQCh. 21 - What is the purpose of a Northern blotting...Ch. 21 - Prob. 21EQCh. 21 - Prob. 22EQCh. 21 - 23. In the Western blot shown here, proteins were...Ch. 21 - If you wanted to know if a protein was made during...Ch. 21 - Prob. 25EQCh. 21 - Prob. 26EQCh. 21 - Prob. 27EQCh. 21 - 28. Describe the rationale behind the...Ch. 21 - Certain hormones, such as epinephrine, can...Ch. 21 - An electrophoretic mobility shift assay can be...Ch. 21 - Prob. 31EQCh. 21 - Prob. 32EQCh. 21 - Prob. 33EQCh. 21 - Prob. 1QSDC
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What is the principle of the SNP (single nucleotide polymorphisms) in the diagnosis of human diseases? a. PCR product of a gene is different from the expected one b. The size of a recombinant DNA is different from the expected one c. Mutation of a single base in a gene makes the size of a band digested by specific restriction enzymes different from the expected one d. The DNA band detected by Southern blot is different from that by Northern blotarrow_forwardWhich of the following is a primary participant in cloning an isolated gene? a. restriction endonuclease b. vector c. host organism d. all of thesearrow_forwardA has been assembled by researchers and transplanted into a donor bacterial strain to study never before seen gene functions. Select one: a. Transgenic genome b. Recombinant DNA sequence c. Knockdown gene d. Synthetic genome o e. Recombinant plasmid Clear my choice is changing our Sequencing the human genome, the development of microarray technology, and understanding of complex diseases like cancer. They help us to observe the gene expression patterns in genetic disease by comparing the healthy tissue of individuals against the disease state of others. Select one: C a. Proteomics o b. Metagenomics MO C. Functional genomics d. Personal genomics O e. Developmental genomics Clear my choicearrow_forward
- Which of the following cannot usually be inserted into a vector to form a recombinant DNA? A. PCR product of a eukaryotic coding sequence B. Lac operon operator region DNA sequence C. Reverse transcriptase gene D. E. coliarrow_forwardSelect the best technique to answer the following question: Where does the transcription factor Nanog bind the rex1 promater? a. EMSA . b. Northern blot . c. DNAse footprinting . d. Bacterial two-hybrid.arrow_forwardDiscuss the process and give example on the following. a. Aptamer Biosensors b. CRISPR c. Stem Cell Therapyarrow_forward
- We have isolated a mutant that changes transcription of many mRNAs, we can use to determine if there are changes to mRNA levels A. microarrays/Sequencing technologies B. Sanger sequencing C. western blot D. all of the abovearrow_forwardWhich of the following approaches you can use to locate the relative positions of genes on chromosomes? A. Recombination-based mapping B. In situ hybridization C. Antibody localization D. Yeast two hybrid O E. Site-directed mutagenesis F. Reporter gene reconstructsarrow_forwardDefine the following terms: a. nonreplicative transposition b. transposable element c. bacterial transformation d. transduction e. conjugationarrow_forward
- Put the following tasks in the order they would occur during a DNA cloning experiment. a. using DNA ligase to seal DNA fragments into vectors b. using a probe to identify a clone in the library c. sequencing the DNA of the clone d. making a DNA library of clones e. cutting genomic DNA with restriction enzymesarrow_forwardHindIII is a restriction endonuclease that recognizes a palindrome sequence. Predict which one is the HindIII recognition site: a. AAGGTT b. AAGCTT c. AGCCGA d. AGCGCA e. AGCAGCarrow_forwardThe Ames Test... a. is always used to detect cancer-causing virus b. has the potential to completely replace the need of animal testing of potential carcinogens c. uses a virus constructed to be susceptible to mutagenesis caused by mutagens d. includes liver extract in the assay to determine the mutagenicity of the metabolites of test samples e. is positive for all human carcinogensarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSON
Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Anatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Infectious Diseases - How do we control them?; Author: Let's Learn Public Health;https://www.youtube.com/watch?v=2JWku3Kjpq0;License: Standard Youtube License