Clonal amplification of DNA using microbiological organism faces challenges associated with (check all that apply): organism toxicity unclonable regions the limited lifespans of microorganisms genomic rearrangements and alterations
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- Drug substances are useful based on their biological activity. Thus, an assay must be developed to ensure that this important function is preserved during purification. There are three major types of biological activity assays including animal model assays, cell-line-derived assays, and in vitro biochemical assays. Please find an example of each (in literature, online, etc.) and briefly Include a reference for each (no particular reference format required, can simply include a web link if easiest).. Throughout downstream processing, various analytical methods must be used to evaluate the effectiveness of unit operations. Provide a method or methods (if more than one is required) for each scenario. Quick determination of the concentration of a purified sample of monoclonal antibody. Determine the concentration of monoclonal antibody in clarified extract. Study aggregation of a protein product without disruption of tertiary protein structure. Characterize phenolics (secondary metabolites and impurities produced by green plant tissue) by identifying phenolic species and relative concentrations.The Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAKO37) requires preparation of a Standard curve: the first time the blanks are run. each time the assay is run. the first time the assay is run. each time the blank is run.
- Secondary LO Select the correct statement/s regarding the application of biotechnology in the field of health and medicine. Question The clones with mutated genes develop on the photographic film due to the complementarity of the probe with the mutated gene in auto-radiography. Option A Preservatives, stabilizers, and adjuvants are added to recombinant DNA vaccines in order to allow the usage of multi-dosage vials, enhancement in storage life, and immune response. Option B If ADA deficiency is detected in the early embryonic stage, then it can be permanently cured by introducing a functional ADA gene Option C into the bone marrow cells. Option Both (B) and (C) Provide Correct Option Herea. Briefly discuss (using three sentences) how the concepts and/or techniques in molecular biology are applied in COVID-19 pandemic, and: b. For each topic (Covid-19 pandemic), give any issues, benefits, or drawbacks of the application (still using three sentences).BiDil® is indicated for the treatment of heart failure as an adjunct to standard therapy in self-identified black patients to improve survival, to prolong time to hospitalization for heart failure, and to improve patient-reported functional status Question: The results of BiDil® clinical trials were compelling…Expound on the results. Question : What role did FDA’s approval resulted in BiDil® being a great example of pharmacogenomics/pharmacogenetics in the U.S.?
- ING SYSTEM (ACADEMIC) estion The use of PCR OLA assay is important for the detection of O a. Flu viruses et ered Ob. HIV infection ed out of Oc. Cystic fibrosis O d. Unknown genetic diseases ag ion O e. Malaria estion Genetic instability in cancers is mainly caused by the following except O a. Microsatellite instability et red O b. Chronic DNA damage d out of O c. Gåining of tumor suppressor genes O d. Deactivation of mismatch repair genes on O e. Gaining of oncogenes stion The most common bacteria species used for the production of indigo dye is Answer: ed d out of stion In the method to prepare the live Cholera vaccine, the truncated A1 peptide was integrated into the ch Cholera strain by? 00 HUAWEI ova 2 Plus DUAL CAMERA edFluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISHElectrophoretic Analysis of DNA via Agarose gel Electrophoresis determine the voltage used (how to determine the voltage? Hint: check on volt/cm).
- Immunofluorescence and Fluorescent livecell imaging techniques can both be used to determine protein localisation.List the advantages and disadvantages of using immunofluorescence andfluorescent live cell imaging10) List down 3 different test animals and give samples for which purposes they can be used in testing of a designed biomaterials? 11) What does FDA abbreviation stand for? 12) Order for the below given steps prior to FDA approval ? Controlled clinical trials In vitro testing (“in glass") In vivo testing w/animal models of disease In vivo testing w/healthy animalsstep 1 Staphylococcus aureus is a bacteria, that is now resistant to commonly used antibiotics, and it is now resistant to the antibiotic methicillin (narrow spectrum beta lactam class). step 2 Describe the physiological mechanism used by individual bacteria to resist the antibiotic listed in above of this Thought Question. For example, the mechanism could be modification of drug, modification of target, prevention of drug penetration, overproduction of target, or target mimicry. (This means that you must choose a strain of bacteria that we know the characteristic of the bacteria that allows the bacteria to be resistant.)