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Topic: Preparation of Microbial Suspension
4. Inaccurate volume measurements of aliquots during dilution.
Effect:
Rationale:
Step by step
Solved in 2 steps
- Topic: Preparation of Microbial Suspension 1. Working in a poorly lit laboratory. Effect: Rationale:Topic: Preparation of Microbial Suspension 5. Over-dilution with sterile water. Effect: Rationale:Topic: Pour-Plate Method 2. Molten agar mixed with bacterial suspension has a temperature higher than 45˚CEffect: Rationale:
- Preparation of Microbial Suspension 1. McFarland Standard was not properly shaken prior to use. Effect: Rationale:Topic: Preparation of Microbial Suspension A Pure colony is needed for inoculation in sterile water, a small portion of culture media was included. Effect: Rationale:TOPIC: BACTERIOLOGICAL EXAMINATION OF WATER a. Does a positive presumptive test mean that the water is absolutely unsafe to drink? Explain.
- Define disinfection. Compare/contrast it with sterilization, antisepsis and bacteriostasis. Give the modes of action of the different antiseptics/disinfectants used in the activity (5% sodium hypochlorite (bleach), 0.05% sodium hypochlorite (bleach), 70% alcohol, 40% alcohol, Iodophor (Betadine), and Mouthwash)Match the physical and chemical methods of control with its use, mode of action etc. Purify H₂0 Sterilize catheters, Petri plates, syringes, sutures placed in plastic wraps Sporocidal antiseptic Their mode of action is lowering the surface tension of water Antiseptic that kills anaerobic bacteria It is use to preserve cadavers, tissues and organs Isopropilic is the most germicidal Standard of disinfectants Choose... Choose... Choose... Choose... Choose... Choose... Choose... Choose... ◆ ◆ → →IN YOUR OWN WORDS. Differentiate the following. (Not more than 10 sentences) 1. Aseptic Teachnique vs. Sterile Technique 2. Microbistatic Agent vs. Microbicidal Agent 3. Sterilization vs. Disinfection 4.How is radiation, autoclave and ultrasonic waves used in the hospitals or clinics as a means to inhibit the growthbof microbes? 5. How is filtration applied nowadays in relation to Covid-19?
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiExamine the given sets of table below. Which disinfectant is the MOST recommended? Support and Justify your answer. Table 2. Phenol Coefficient of Test Disinfectant Name of disinfectant Growth after 5 minutes Dettol Phenol Name of disinfectant Z-germicSide Phenol Name of disinfectant Jik Phenol KEY:- = No growth, + = Growth Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 Dilution 1:100 1:125 1:150 1:175 1:200 1:95 1:100 1:105 1:110 1:115 + + + + + + + + Growth after 5 mins + + + + + + + Growth after 5 mins + + + + + + Growth after 10 minutes + + + + + Growth after 10 minutes + + + + + Growth after 10 mins + - + + + + + + Growth after 15 minutes + + + + + + Growth after 15 mins - + + + + + Growth after 15 mins + + + + - + + + +Match the most appropriate method of sterilisation for the following items. 10 mL vitamin solution 5 mL antibiotic solution Petri dishes 1 L of water 1 L of nutrient broth medium Talcum powder Charcoal powder [Choose ] [Choose ] Gamma radiation Autoclave Dry heat oven Filtration [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]