Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
expand_more
expand_more
format_list_bulleted
Concept explainers
Question
Chapter 17.5, Problem 1TQ
Summary Introduction
To review:
The identification of Gram-negative bacteria which is 1µm (micrometer) wide, straight, motile, does not have sheath, and contains sulfur granules.
Introduction:
The nomenclature and classification of organism is based on their genetic relatedness. In addition, several other characteristics like
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solutionStudents have asked these similar questions
Epulopiscium fishelsoni is a Gram-positive bacteria found in the gut of a sturgeon fish. The largest
ones found are up to 700μm in length. Your field of view when using the 100X objective has a
diameter of 12mm. If you were to line up your Epulopiscium fishelsoni in a strepto- fashion, how
many entire bacteria would fit in your field of view? Write just the number with any needed
commas. Do not include units.
A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate.
How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2.
Since only 0.1 mL is put on the plate, this counts as an extra dilution!!!
Any time less than 1 mL is transferred, a dilution is being performed.
Any time more than 1 mL is transferred, a concentration is being performed.
Include the trailing zero so there are two decimal places
Canvas expects a single digit before the decimal point.
5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation
54.00*10^1 would be marked wrong.
0.54*10^3 would be marked wrong.
A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…
The following data were obtained by incubating gram-positive bacteria in nutrient medium +
disinfectant for 24 hours, then transferring one loopful to nutrient medium (subculturing). (+ =
growth; - = no growth.
a. Table 7.3, which disinfectant is the most effective at stopping bacterial growth?
How can you tell?
b. In Table 7.3, which disinfectant was bactericidal? How can you tell?
c. In Table 7.3, which disinfectant was most effective against Salmonella? How can
you tell? [Hint: look up Salmonella]
Table 7.3
K.O.
Subculture
Doom
Dilution
Initial
Subculture
Initial
1:16
1:32
1:64
1:128
Mortum
Sterl
Dilution
Initial
Subculture
Initial
Subculture
1:16
1:32
1:64
1:128
Chapter 17 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- 1) Listen In the lab you are testing a bacterium's oxygen requirement for growth. You grow your bacterium in a test tube, without shaking at the appropriate temperature. The next day you find that your bacterial culture is growing only at the top surface (ie at the top of the tube closest to the cap) and not dispersed throughout the test tube. What term would be used to describe the oxygen requirement of this bacterium? O A) Obligate aerobe. O B) Microaerophile. O C) Anaerobe. D) Obligate anaerobe. E) Facultative anaerobe.arrow_forwardThe peptidoglycan layer in a Gram-negative cell is typically .008 um thick while the peptidoglycan layer in a Gram-positive cell can be up to 0.00000008 m thick. Which cell type has a thicker layer of peptidoglycan. The Gram negative cell is 1,000x thicker than the Gram positive cell. The Gram positive cell is 1,000,000x thicker than the Gram negative cell. The Gram positive cell is 1,000x thicker than the Gram negative cell. The Gram negative cell is 1,000,000x thicker than the Gram positive cell. The Gram negative cell is 10x thicker than the Gram positive cell. The Gram positive cell is 10x thicker than the Gram negative cell.arrow_forwardYou have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted35 colonies. What was the cell density of your original culture (in cells/ml)? How many cellsdid you have in 100ml of that culture? DON’T FORGET TO ROUND!arrow_forward
- Assume that after washing your hands, you leave ten bacterial cells on a new bar of soap. You then decide to do a plate count of the soap after it was left in the soap dish for 24 hours. You dilute 1 g of the soap 1:106 and plate it on heterotrophic plate count agar. After 24 hours of incubation, there are 168 colonies. How many bacteria were on the soap? How did they get there?arrow_forwardUsing a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units. If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu? Express your answer in scientific notation rounded to two decimal places. Eg. 0.01001 would be 1.00*10^-2 You must include trailing zeros 0.0000040001 would be reported as 4.00*10^-6 There must always be two decimal places, even if they are zeros Canvas expects one digit before the decimal point 0.40*10^-5 would be marked incorrect for 0.0000040001arrow_forwardYou use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x 10^5 cells/ml. Assuming you will plate 0.1ml of your samples, to what factor should you dilute your sample to get a countable number of colonies? Rearrange Original Cell Density formula to solve.arrow_forward
- Why is E. coli O157:H7 an organism of concern in contaminated foods? The strain is represented as O157:H7. Which particular “parts” of the bacterial cell do the “O” and “H” refer to?arrow_forwardYou prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and counted 67 cfu. What was the cell density of your bacterial culture (in cfu/ml? How many cells did you have in total if the volume of your culture was 50ml? Round to a whole number, do not write in scientific notation. The cell density of my bacterial culture was cfu/ml. The total number of cells wasarrow_forwardYou were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)arrow_forward
- Assume that you counted 67 plaques on a bacterial plate where 0.1ml of a 10-5 dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage? Show your calculations and give your answer in pfu/ml (pfu = plaque-forming units)arrow_forwardCalculate the surface/ volume ratio of the spherical Neisseria gonorhoe bacterium. Compare this with the surface -to-volume ratio of the large eukaryote ameba Neisseria gonorhoe bacterium = 0.5 micrometer in diameter eukaryote ameba = 150 mm in diameterarrow_forwardYou perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 mlarrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
Bacterial Structure and Functions; Author: Osmosis;https://www.youtube.com/watch?v=b15Hy3jCPDs;License: Standard youtube license