Chickpeas Temperature Experiment

In this experiment, I will be comparing the metabolic rates of two different species. Because they are two different species, and differ in physiology, I will expect to see a difference in metabolic rate. More specifically, I expect crickets to have a higher metabolic rate, due to their efficient respiratory system.

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

Our data recorded shows that the germinating peas did consume more oxygen than the non-germinating or the glass beads alone and that the cooler temperature did slow down the consumption of oxygen in the germinating peas. In both water baths the atmospheric pressure seemed to increase causing our reading to raise in our glass beads and non-germinating peas. This direct relationship in reading leads us to believe that the oxygen consumption in the non-germinating peas was minimal if any at all.

The first lab was conducted to analyze how germination affects the rate of cellular respiration in lima beans compared to dormant seeds. In order to

direct calorimetry. Furthermore, the amount of oxygen in the chamber reveals the amount of cellular respiration of the organism. While also, test the effects of decreasing oxygen, and later increasing the heat on the metabolic rate of goldfish. I hypothesize that an increase in temperature will increase their metabolic rate

There are many procedures during this lab and many materials needed for an accurate analysis of data. First, fill a 100 mL graduated cylinder with 50 mL of water. Add 25 germinating peas and determine the amount of water that is displaced. Record this volume of the 25 germinating peas, then remove the peas and put those peas on a paper towel. They will be used for the first respirometer. Next, refill the graduated cylinder with 50 mL of water and add 25 non-germinating peas to it. Add glass beads to the graduated cylinder until the volume is the same to that of germinating peas. Remove the beads and peas and put on a paper towel. They will be used in respirometer 2. Now, the graduated cylinder was filled once again, determine how many glass beads will be require to reach the same volume of the germinating peas. Remove the beads and they will be used in respirometer 3. Then repeat the procedures used above to prepare a second set of germinating peas, dry peas and beads, and beads to be used in respirometers 4,5,and 6, the only difference is the temperature of the water.

2% Sodium Hydrogen Carbonate (5g per trial) will be used to supply the carbon dioxide needed for photosynthesis

There were three test tubes in which the experiment was held. A relatively equal sized portion of raw potato (this contained the enzyme [a biological catalyst] hydrogen peroxidase) was placed in each tube. Then, enough water to cover the potato was added. Proceeding this, each of the test tubes were assigned a temperature; cold, room temperature or warm (this was written on the tag so that they were not confused). The test tube destinated ‘cold’ was placed in a ice bath for five minutes. At the same time, the ‘hot’ test tube was placed in a hot water bath for five minutes. Meanwhile, the room temperature test tube sat at room temperature for five minutes. When the five minutes were over, the test tubes were returned to the rack (so that they were able to be observed). Then, the test tubes were allowed to sit at room temperature for five more minutes. Once that period of time was over, 2 ml of hydrogen peroxide (the substrate) was added to each tube.

Respiration was measured in germinating seeds in the Pea Lab because in germinating peas there is a high rate of cell respiration as the pea is still growing and needs to consume oxygen to continue growing. Pea plant cells rely on the process of cellular respiration to supply them with the energy that they need to stay alive and grow during germination. When the pea plants are grown the cells will still remove energy from sugar via cellular respiration; however, the sugar for cellular respiration will come from the process of photosynthesis instead of the stored

Meanwhile, the peas did carry out cellular respiration. Thus by correcting the readings from the peas with the readings from the beads, it would should the actual rate at which cellular respiration will occur in peas through not allowing changes in outside conditions (i.e. atmospheric pressure) affect the respiration rate. By correcting the readings, it will show the most accurate results under the conditions of the lab without any changes affecting the

The procedure for this experiment was to first obtain four balloons and blow them up in order to stretch them. Then obtain and fill the four large test tubes each with thirty milliliters of warm forty degrees Celsius water and two grams of dry yeast which was weighed on a scale and scooped out by a spatula. After five milliliters of water, ten percent glucose, fructose or sucrose went into one of the four test tubes. Then parafilm was placed on top of each of the test tubes to seal them and they were swirled activating the yeast through rehydration. After swirling the film was removed and the balloons were tightly placed on the test tubes. Then finally observed the tubes build up of CO2 all the while swirling gently every fifteen minutes, recording observations.

The change in temperature affects the respiration of goldfish. In goldfish, active metabolic rate decreases as temperature is lowered (Johnston and Dunn 1987). The goldfish breathes per minute is decreased as oxygen is required for this activity. A similar experiment conducted supports the result from this experiment that oxygen consumption decreases as temperature decreases (Fry and Hart 1948). The decrease in breathes per minute was observed as the temperature was lowered. The average breathes per minute decreased from 110 to 92 per minute when the temperature was lowered from 22 to 12. So, the null hypothesis that the change in temperature will not affect the breathes of the goldfish was rejected. There were some sources of error for this

Cellular respiration is an important life process that is very detailed and can be affected by several factors, one which being temperature.(particle movement) As time went on, more oxygen was consumed by the germinating seeds, indicating a quicker rate of respiration. As I hypothesized the germinated seedlings underwent the highest cellular respiration rate at 30 °C. Based on the data, it can be concluded that germinating radish seeds have a higher rate of cellular respiration in warmer

This experiment consisted of 3 respirometers, one with ants, one with radish seeds, and one with glass beads. Each with 4 pellets of KOH and a piece of cotton. They were placed in a water bath that was at 75 degrees fahrenheit. A bubble at the end of the respirometer was measured every five minutes, and this distance showed how well the organisms were respiring. The radish seeds were able to do the most cellular respiration in 25 minutes, with the ants being a close second, and the control respirometer of the glass beads doing the least.

To find the effect of temperature on the activity of an enzyme, the experiment deals with the steps as follows. First, 3 mL if pH 7 phosphate buffer was used to fill three different test tubes that were labeled 10, 24, and 50. These three test tubes were set in three different temperature settings. The first test tube was placed in an ice-water bath for ten minutes until it reached a temperature of 2° C or less. The second tube’s temperature setting was at room temperature until a temperature of 21°C was reached. The third tube was placed in a beaker of warm-water until the contents of the beaker reached a temperature setting of 60° C. There were four more test tubes that were included in the procedure. Two of the test tubes contained potato juice were one was put in ice and the other was placed in warm-water. The other two test tubes contained catechol. One test tube was put in ice and the other in warm water. After