1. Calculate the pH of the following solutions a. 2 μM HCI b. 3 x 10-³ M KOH
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- At a pH of 7.40, the carbonic acid ratio is ________. a. 35:1 b. 4:1 c. 20:1 d. 3:11. Given .750L of a 12M HCI solution, what volume of 5.00M HCI could you make?1) Calculate the required gram of Tris-Hcl to prepare 1M, 100 ml Tris-HCl (Tris Stock) with mol and Molarity formula. 2)Calculate the required gram of EDTA to prepare O.5M, 100 ml EDTA (EDTA stock) mol and Molarity formula. 3.) Calculate the required volume of Tris Stock to prepare buffer A which contains 25 mM Tris-Hcl with final volume is 100 ml by using M1V1 =M2V2 formula 4) Calculate the required volume of EDTA stock to prepare buffer A which contains 10 mM EDTA with final volume is 100 ml by using M1V1 =M2V2 formula 5)Do the same for Buffer B calculations
- 1. Calculate the pH of the following solutions: A. [H+] = 1.36 x 10-9 M В. [ОН] —D 3.86 x 10-8 М 2. Calculate the pOH of the following solutions: A. [OH-] = 8.6 x 10-8 M В. рH %3D 3.2 3. What is the pH of a buffer prepared by mixing 100 ml 0.050 mM NAH2PO4 and 25 ml 0.075 mM Na2HPO4? (рКa1%32.2; рКо23 7.21; pКаз312.7)1. What is the concentration of the solution prepared by diluting 50 µl of ampicillin (1.0mg/ml) to a final volume of 1ml? 2. Determine the volumes of phenol, chloroform, and isoamyl alcohol required to prepare250 ml of a 25:24:1 (v/v) solution.Given a BSA stock solution of 4mg/mL how would you prepare a 5 mL sample with a final concentration of 1 mg/mL?
- What is the approximate pH of a solution labeled 6 x 10-5 M HBr? * 4.2 4.5 5.8 9.8 8.21. What mass of potassium nitrate KNO3 should be added to 255 g of water to prepare a 15% (m/m) solution of KNO3? [Molar mass KNO3= 101.1 g/mole] 2. Determine the mass of sodium chloride (NaCl) required to prepare a 300 ml Mueller-Hinton broth solution supplemented with 5.5% (wt/vol) sodium chloride solution. [Mwt NaCl = 58.44 g/mol] 3. You have dissolved 45 g of magnesium sulphate in 300 ml distilled water (dH2O). What is the concentration of this MgSO4 solution expressed as % (m/v)? [Mwt MgSO4 = 120.37 g/mol]1. Prepare 30 mL of a 5 mg/mL oral phenobarbital solution using phenobarbital available from the manufacturer in a 65 mg/mL solution that comes in 1-mL vials. How much stock solution will be required? 2. What mass in milligrams of potassium nitrate is present in 0.25 kg of a 500 ppm aqueous solution of KNO,?
- H2SO4 is 0,5 mol What is the pH of this solution? What is the pH of 35 ml buffer solution which is 0,3 M in CH3COOH and 0,5 M in CH3COONA ? 7. The pH of buffer solution is 7.0. What is the hydronium-ion conor1. What volumes of 50 mM CH3COOH and 30 mM NaCH3CO0 solutions are needed to prepare 2L of 20 mM acetate buffer pH 3.6? (pka for acetic acid is 4.74)50X TBE is 87 mM Tris-borate and 4 mM EDTA at pH 8.3. What is the molarity of each of the chemicals in 1X TBE buffer?
