1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure?
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Polymerase Chain Reaction
Questions:
1. What are the materials used for the polymerase chain reaction?
2. Draw a schematic diagram of the procedure in PCR.
3. Why is it important to design the primers at the start of the laboratory procedure?
4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer?
5. What is the use for magnesium chloride?
6. How much template DNA is added? What is the concentration of the primers?
7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature?
8. In this particular PCR experiment, how many cycles was used?
9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
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