24. The diagram below shows the map for EcoRI restriction sites around the BRCA1 gene for two alleles of the gene, andthese alleles. The DNA was digested with the restriction enzyme EcoRI and probed with a radioactively labeled DNA froma cloned copy of the BRCA1 gene. Which individual or individuals are homozygous for allele 1?a southern blot of DNA for four people that are either homozygous for one of the two alleles or are heterozygous foSouthern Blot12 34Allele 1Allele 2BRCA1EcoRIEcoRIEcoRI10 kbLA) 1B) 2 and 3C) 412 kbD) 1 and 4E) You cannot identify homozygous individuals without data for the offspring of these individuals.==

Answered: 24. The diagram below shows the map for…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...

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#16) The restriction enzymes Xhol and SalI cut their specific sequences as shown below: XhoI 5' C | TCGAG 3' SalI | 5' GTCGAC 3' 3' GAGC | TC 5' 3' G | AGCTG 5' Can the sticky ends created by XhoI and SalI sites be ligated? If yes, can the resulting sequences be cleaved by either XhoI or SalI?
1a.The complete genotype of the host yeast strain is MATa ade2 his3 leu2 met15 ura3. Name one advantage of using a yeast strain with auxotrophies for several genes (i.e. his3, leu2 and ura3). b.)After successful CRIPSR editing, the yeast can later be “cured” of the recombinant CRISPR plasmid – that is, the plasmid is lost but the CRISPR edit is stably inherited. Write the new genotype of the yeast based on ADE6 gene.
Refer to Figure , which describes the base modifications of bacteriophage T4 DNA, and briefly describe some issues that must be dealt with in preparing a restriction map of T4 DNA.
1. A DNA sequence 10 kb long contains one site at which it may be cut by the restriction enzyme Pstl. The same 10 kb long sequence also contains one site that can be cut by restriction enzyme Bg/II. Three separate fractions of this DNA were treated separately, as follows: 1) digestion by Pstl; 2) digestion by Bg/II; 3) digestion by both enzymes. The three treated fractions were then subjected to gel electrophoresis. The following table shows the lengths of the restriction fragments associated with each treated fraction. From the result, draw a restriction map that shows the Pst and the Bg/II cutting sites in the 10 kb DNA. Enzyme in mixture Pstl BgIII Pstl and BgIII Fragment Lengths (kb) 6.4 and 3.6 8.8 and 1.2 5.2, 3.6 and 1.2
The drawing below depicts a restriction map of a segment of a human chromosome. The cut sites shown (1 through 6) are known to be polymorphic and can be present or absent sending upon the genetic makeup of a specific individual's chromosomes. E identifies Eco RI sites and P identifies Pst 1 sites. The line below the map identifies the region of homology between the DNA and a probe used in Southern blot analyses to characterize this region. A. Individual 1 has restriction sites 1-6 (all) on both copies of this chromosome. If DNA from this individual was digested with Pst I, what are the sizes of the fragments that would be detected via Southern blow using the identified probe? B. DNA from individual 1 is digested with both Eco RI and Pst I and then analyzed by Southern blot. What bands are revealed? C. Individual 2 is homozygous for a polymorphism that eliminates site 3. If DNA from this individual was ingested with both Pst I and Eco RI, what are the sizes of the fragments that would…
A B Figure 1 The postgraduate student, Vanessa, cloned her gene of interest into two different vectors; pUC18 and pZERO®-1, and transformed the competent cells with the recombinant vectors. After that, she plated them on the antibiotic plates and incubated her samples for 18 hours. The next day, she realized that the plates were not labelled. (i) Based on the observation above, can you identify which plate is carrying the recombinant pUC18 and pZErOⓇ-1, respectively? (ii) Why are there small colonies surrounding the big colonies in Figure 1 (A)? Explain your answer in detail. (iii) Do you think all the colonies present on the plates in Figure 1 (B) contain the gene of interest? Describe the selection procedures for recombinant pZErO®-1.
1. Briefly explain why the total size of the pMBBS plasmid in the Restriction Enzymes practical is 3000bp (base pairs) 2. Briefly explain why the cut sites on the pMBBS plasmid in each Restriction Enzymes (EcoRI, BamHI, and XhoI) just 1 each 3. Briefly explain what led to the 5 fragments formed which linked to the 500bp, 1000bp, 1500bp, 2000bp, 2500bp sizes
This is a restriction map for the 250 base pair plasmid pSage. Restriction sites for the restriction endonuclease Nhel are 7, 69 and 160. What are the sizes of the restriction fragments produced? Check all that apply. p SAGE Nhel 7 250 bp Nhền 160 Nhel 69 62 69 91 160 97
a. Why doesn't Dpnl digest the mutagenized DNA? b.What is the approximate size, in base pairs, of the pQE.1-CRYGD plasmid?
a. Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize it into a plasmid. b. What are restriction length polymorphisms, and how are they used?
a. How do scientists apply the concept of linkage disequilibrium to identify disease alleles? b. Which specific phrase is used when such markers are identified by restriction endonucleases and a particular set of DNA fragments is generated?
#2.) Mary wants to further confirm if she is a carrier for this lethal gene. She was told that a new SNP marker was identified RFPL marker. She requested to do SNP test for all the DNA samples. The results are: her parents are heterozygous for SNPs (that is to say, one chromosome is wild-type and another one contains the SNP). The sister who passed away is homozygous for the SNP. Mary does not have this SNP. So now what is the probability of Mary as a carrier of the death gene?

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