ChemistryHi, i just need some help on how to calculate the concentration of the samples? Thank you Table 2: Nelson Glucose Assay – volumes and sequence for addition of reagentsas well as glucose concentrations and absorbances for standards andsample tubesDeprot.samplesGlucose StandardsAВDE#1#2Standard/samples ()0.20.22 Nelson's copper (mL)0.50.53 Arsenomolybdate mL)0.50.54 Distilled water (mL)0.70.7Total volume (mL)1.91.9Glucose conc. (µM)75150225300Absorbance (600 nm)0.06540.2119 0.3097 0.4397 0.247610 0.0722

The question involves the beer lambert law equation: A=ε c lA is the absorbance; c= concentrationl =…

Answered: ample tub Deprot. samples Glucose…

Chemistry: Principles and Reactions

8th Edition

ISBN:9781305079373

Author:William L. Masterton, Cecile N. Hurley

Publisher:William L. Masterton, Cecile N. Hurley

Chapter10: Solutions

Section: Chapter Questions

Problem 6QAP

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Chemistry

Hi, i just need some help on how to calculate the concentration of the samples? Thank you

Table 2: Nelson Glucose Assay – volumes and sequence for addition of reagents
as well as glucose concentrations and absorbances for standards and
sample tubes
Deprot.
samples
Glucose Standards
A
В
D
E
#1
#2
Standard/samples ()
0.2
0.2
2 Nelson's copper (mL)
0.5
0.5
3 Arsenomolybdate mL)
0.5
0.5
4 Distilled water (mL)
0.7
0.7
Total volume (mL)
1.9
1.9
Glucose conc. (µM)
75
150
225
300
Absorbance (600 nm)
0.0654
0.2119 0.3097 0.4397 0.247610 0.0722

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