Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's Law
Q: Why is it important to use standards or to develop a standard curve in spectrophotometry?
A: Spectrophotometry is the analytical technique by which the character of a substance is measured…
Q: Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron,…
A: There are different types of microscopy like bright-field, phase-contrast, dark-field, fluorescence,…
Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.
A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…
Q: What is the diameter of field of view for the high-power objective in millimeters and micrometers?
A: A microscope is a device that allows the examining of small objects, including cells. The picture of…
Q: Draw and label FIVE (5) main components for UV-Vis spectrophotometry. Briefly explain the function…
A: When Lab scientists have to be measured total protein by UV-spectroscopy some important things…
Q: What total magnification will be achieved if the 10× eyepiece and the 100× objective are…
A: The microscope is an instrument which used to view and observe tiny organism or an object that…
Q: What is Audiometer? When is it used?
A: An Audiometer is a subjective device that is used to evaluate the hearing threshold of a person. It…
Q: What is the diameter of the scanning field of view in millimeters? ___________
A: The microscope is an instrument which used to view and observe tiny organism or an object that…
Q: Complete the following table by calculating the total magnification of the different objectives of…
A: Microscope is used to visualize objects that are not visible to the naked eye. It consists of…
Q: WHAT ARE THE Steps in spectrophotometer
A: -Spectrophotometer is an optical instrument which is used to measure the concentration of a known…
Q: What are the differences between scanner, low power objective, high power objective and oil…
A:
Q: What are the light sources used in UV-vis spectrophotometry
A: Spectrophotometer is an instrument used for measuring the concentration of solutes in solution by…
Q: Suppose you were in a research lab in Romania and the lab is equipped with several…
A: Proteins are biomolecules that play a variety of roles in living systems. They include catalysis,…
Q: Explain the reason for the use of different medium blanks in adjusting thespectrophotometer prior to…
A: Different medium for the growth of bacteria have different components. Due to presence of different…
Q: Total Magnification is obtained by Select one: A. Magnifying power of both the objective lens and…
A: Magnification is the term used for the increase in the size of the sample when seen under a…
Q: Differentiate between the limit of resolution of the typical light microscope and that of the…
A: Microscope is a instrument which is used to observe the minute organisms and the processes occurring…
Q: What is shown? Why did we use the slide? What is the arrow pointing to? What did we use it for? What…
A: Microscope is a instrument which is used to visualise the specimen that can't be seen by naked eyes…
Q: Which of the following is true about the relationship between depth of field and magnification?…
A: The depth of field refers to resolution in the longitudinal plane. It is measured as the distance…
Q: What is the relationship between the working distance of an objective lens and its magnification…
A: Microscope is an optical instrument that uses a lens or an arrangement of lenses to magnify an…
Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…
A: A microscope is an instrument used in laboratories to examine tiny objects that cannot be visualized…
Q: If the total magnification is 2000X with the use of a 10X ocular lens, what is the magnification of…
A: Answer- 200X
Q: Listen Name the Medical Imaging modalities, which utilizes ionizing radiation.
A: The application of ionizing radiation in medical imaging is very vast.The advantage of using…
Q: Explain how total magnification is calculated. Select an answer. a. It is the magnification of the…
A: An instrument that creates an enlarged image of a small object, thus revealing details too small to…
Q: An object is 20µm in diameter. If viewed through a 10X ocular lens and a 40X objective lens, how…
A: Answer: MICROSCOPY : It is a technique for making small things to visible by magnifying through…
Q: What is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x…
A: Given: Objective lens 1 magnification= 1000x DFV1 = ? Objective lens 2 magnification= 400x DFV2 =…
Q: principle of basic spectrophotometer
A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…
Q: Calculate the diameter of the field of view for each total magnification on your microscope in…
A: Field of view (FOV) is the diameter of the circle of light that can be seen while looking into the…
Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…
A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…
Q: How can one obtain 2,000× magnification with a 100× objective?
A: Magnification means how many times the image is larger than the object.
Q: Question 1 options: Length of the sample tube Concentration of the substance Temperature…
A: The property of the compound to rotate the plane of polarization of the plane polarized light is…
Q: Define each of the following terms: A) What is resolution and how is resolution related to the…
A: According to the question, we have to provide the solution of the questions one by one. These…
Q: What is the main application of Atomic absorption spectrophotometer (AAS)?
A: The Atomic Absorption Spectrophotometer (AAS) is primarily used to quantify the presence of several…
Q: Give two applications of Spectrophotometric analysis and briefly explain each
A: The spectrophotometer is an instrument that uses an electromagnetic spectrum. This instrument is…
Q: What are the functions of the Fine Focus, Coarse Focus, Stage Clip, Rack Stop, Arm, Eyepiece,…
A: A microscope is considered a laboratory instrument to observe microorganisms, small objects, cells,…
Q: What solution goes into the cuvette when you’re zeroing the spectrophotometer?
A: Spectrophotometer is a device that is used to calculate the absorbance of the light passing through…
Q: If the total magnification of a slide is 400x and the ocular lenses are 10x, what is the magnifying…
A: Magnification is the process of enlarging the apparent size. This enlargement is quantified by a…
Q: Introduce and explain comprehensively the process of calculating the final magnification of…
A: A microscope is a device that magnifies an object so that the spectator can see it. A microscope is…
Q: Do the calibration factor computed varies according to different magnification (objective lenses…
A: By simply accounting for the inaccuracy, the Correction Factor allows us to attain more accurate…
Q: spectrophotometry, what is the role of the green background in the set-up? What does it represent in…
A: Introduction:- Spectrophotometry is a technique for determining how much light a chemical substance…
Q: what objective lens is the oil objective lens?
A: We have to determine the objective lens that is used for oil immersion.
Q: If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x,…
A: According to the question, the total magnification of a microscope is 700X.…
Q: Explain the working principle of Atomic Absorption Spectrophotometer and Draw the general Diagram of…
A: Atomic absorption spectrometery is highly practiced to analyse compounds in the solutions and this…
Q: What is the principle behind a UV-Vis spectrophotometer, and what are its key components, and how…
A: UV-vis spectrophotometry or UV spectroscopy types of absorption spectroscopy or reflectance…
Q: Question 5 What is the total magnification of a sample with an ocular lens power of 15X and using a…
A: A compound microscope has two lens one is ocular or eyepiece lens and objective lens. Objective lens…
Q: What is Magnification?
A: The instrument microscope aims at visualizing the magnified view of the objects/organisms that are…
Q: Why do you need to move the lever of the aperture diagram all the way to the left (smallest value)…
A: DOING THIS WILL HELP PREVENT EYESTRAIN. ONCE THE SPECIMEN IS IN FOCUS, IT IS TIME TO ADJUST THE…
Q: What is the stationary and mobile phase of spectrophotometry?
A: Introduction A biotechnique based on the principle of quantitative measurement of light intensity…
Answer the following questions:
1. Give the general steps in the use of a spectrophotometer.
2. Define the following:
a. ) Optical Density
b.) Transmittance
c.) Beer's Law
Step by step
Solved in 2 steps
- Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?Explain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.Explain the underlying physical principles in the creation of image contrasts for the following two processes of image contrasts for the following two procedures. (a) Magnetic resonance tomography (b) X-ray-computed tomography
- What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?If we wanted to know how “red” a red liquid solution is, we would use a light transmittance measurement. Based on the lab, what device would be used? a) manometer b) chromatogram c) spectrophotometer d) manometer and spectrophotometerWhat are the light sources used in UV-vis spectrophotometry.give ans
- Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %Identify all accurate statements: Using a neutral excitation filter can reduce photobleaching. However… A.This can reduce the fluorescence emitted. B.This only delays photobleaching if exposure to light is prolonged. C.This can introduce aberrations in the image. D.This may be toxic to the sample to be observed.Name two radioisotopes used within the medical imaging processes, one administered by inhalation and one used by intravenous injection. For both of these radioisotopes, what are their half-life and what is the relevance of that to why it is used? What are both of these specific use in diagnostic imaging?What are they treating?
- Using the Field of View (FoV) formula: (Low Magnification/High Magnification)= (High Field of View/Low Field of View) determine the FoV in microns for all other objective lenses (4x,10x,40,100x) given that the diameter of the 4X scanner FoV is 3.2 mm Please, explain with the formula how it was used and how values were addedIn spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?For what purpose would you adjust each of the following microscopecomponents during a microscopy exercise?a. Iris diaphragm:b. Coarse-adjustment knob:c. Fine-adjustment knob:d. Condenser:e. Mechanical stage control: