Describe how the SCAM analysis was performed so that it maintains replicatability. kDa55 Cysless352010F16CE39CL860G125C+++UM18CV51CY96CF139CM36CK72C---+ MTSES+ - NEM+ + + MPEG5K|D106C+P←UR143C+P←U+ MTSES- NEM+ MPEG5K+P+U+ MTSES- NEM+++ MPEG5K+P←U+ MTSESNEM+++ MPEG5KFig. 1 SCAM analysis of AlaE. Western blot analysis of whole celllysates from cells harbouring plasmids expressing cysteine-free('Cysless') or single cysteine variants of alaE with and without treatmentwith NEM, MTSES and mPEG5K. PEGylated (P) and unmodified proteinare indicated with arrows.

SCAM (Substituted Cysteine Accessibility Method) is an analytical technique which is used to study…

Answered: Describe how the SCAM analysis was…

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Describe how the SCAM analysis was performed so that it maintains replicatability.

Biology 2e

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ISBN:9781947172517

Author:Matthew Douglas, Jung Choi, Mary Ann Clark

Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark

Chapter35: The Nervous System

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Question

Describe how the SCAM analysis was performed so that it maintains replicatability.

kDa
55 Cysless
35
20
10
F16C
E39C
L860
G125C
+
+
+U
M18C
V51C
Y96C
F139C
M36C
K72C
---+ MTSES
+ - NEM
+ + + MPEG5K
|D106C
+P
←U
R143C
+P
←U
+ MTSES
- NEM
+ MPEG5K
+P
+U
+ MTSES
- NEM
+++ MPEG5K
+P
←U
+ MTSES
NEM
+++ MPEG5K
Fig. 1 SCAM analysis of AlaE. Western blot analysis of whole cell
lysates from cells harbouring plasmids expressing cysteine-free
('Cysless') or single cysteine variants of alaE with and without treatment
with NEM, MTSES and mPEG5K. PEGylated (P) and unmodified protein
are indicated with arrows.

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