draw the alkane strucutre based off the IR spectrum, 1H NMR spectrum, and 13C NMR spectrum for this compound.
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- 80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.3 Acra Alrich TOI NOF 200 250 300 350 Wavelength (nm) UV/Vis spectrum of four brands of polysorbate 80, a common detergent used in protein purification. From Wuelfin, et al., "Polysorbate 80 UV/vis spectral and chromatographic characteristics-defining boundary conditions for use of the surfactant in dissolution analysis." 3. While the binding of Coomassie blue to proteins can lead to accurate measurements of protein concentration, there are situations where the absorbance values could be misleading. For example, detergents are often used in the purification of proteins. Certain detergents mixed in with the dye could lead to incorrect determinations of protein concentration. Take a look at the spectrum of the detergent polysorbate 80, above. If you had used this detergent to purify the protein in part c above, would you expect it to interfere with your concentration calculations? Why or why not? Absorbance (AU)20.28 The following initial velocities were determined spec- trophotometrically for solutions of sodium succinate to which a constant amount of succinoxidase was added. The velocities are given as the change in absorbancy at 250 nm in 10 s. Calculate V, KM, and K, for malonate. AX 103 10 s 15 x 10-6 mol L-1 [Succinate] 10-3 mol L-1 No Inhibitor Malonate 10.0 16.7 14.9 2.0 14.2 10.0 1.0 11.3 7.7 0.5 8.8 4.9 0.33 7.1 Hint: Treat this problem using the competitive inhibited rate law
- A flat (not spherical) tissue engineered skin (thickness 3.9 mm) is attached to the bottom of a dish and cultured with cell media atop it (which has an oxygen concentration of 0.15 mol/m3). The diffusivity of oxygen in the skin is 3.2 E-9 m?/s, and according to experiments that you've performed, you want to make sure that the concentration of oxygen in the device doesn't fall below 0.06 mol/m3. (Below that concentration harms the cells.) The rate of oxygen consumption is -5 E-17 mol/[cell•s]. What is the maximum cellularity (in cells/mL, three significant digits) that this tissue engineered skin can support?Paper electrophoresis of Asn, Ala, Asp, Lys and Ser mixtures at pH = 7 shows the fastest movement towards the anode The fully saponified 10g oil sample consumed 1.87g KOH, and the iodine value of the oil was 28.3. So how many unsaturated double bonds are there in each oil molecule on average? (lodine is known to have an atomic weight of 127) Given that 100g cellulose sample is completely hydrolyzed to obtain 78g glucose, then the percentage of cellulose in the sample is _% Given that the Km of catalase is 20mmol/L and the substrate concentration is 80mmol/L, then the percentage of catalase bound to the substrate is %Specific rotation of L-alanine [(+)alanine) in water (at 25 °C) is +2.8. Suppose you have 2.00 g of mixture of L and D-alanine [(-)alanine]. You dissolved it in 10 mL of water, and measured rotation in 10 cm cell. The observed rotation was +0.12. Calculate the ee% for the mixture. How many grams of D- and L-alanine do you have in the sample?
- 2. 60mL of a 0.2M solution of tetrapeptide Cys-Met-Arg-Asp is adjusted to pH=8. The solution is then titrated with 90mL of 0.2M HCI. a. Calculate the new pH of this solution. (Relevant pKa values are 2.1, 3.8, 8.3, 9.6, and 12.5) b. After the addition of HCl in part a, the solution is then titrated with 50mL of 0.3M NaOH. What is the final pH of this solution?At Electrochemical Equilibrium, which best describes the distribution of ions in the chamber, on either side of the membrane, when one "mole" of salt (NaCl) is added to one side of a 1 liter chamber, filled with water and divided in half by a membrane permeable only to Na+. 2.0 M CI- and 1.25 M Na+ one side of the membrane; 0.75 M Na+ on the other side of the membrane. 1.0 M CI- and 1.0 M Na+ on both sides of the membrane. 2.0 M CI- and 1.0 M Na+ one side of the membrane; 1.0 M Na+ on the other side of the membrane. 2.0 M CI- and 2.0 M Na+ on one side of the membrane; pure water on the other side of the membrane.MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?
- at 257 nm) is dissolved in 2.5 mL of buffer. A 250 microliter aliquot is removed and placed in a 1.0 cm A sample of adenosine triphosphate (ATP) (MW 507, ɛ = 14,700 M'cm cuvette with sufficient buffer to give a total volume of 2.0 mL. The absorbance of the sample at 257 nm is 2.0. Calculate the weight of ATP in the original 2.5 mL sample. (Choose the correct value from the drop-down list provided. The values of weight are listed in units of milligrams.) Weight of ATP: v mg10 tab of acetaminophen 500 mg molar absorptivity= 14520 @ 243nm. The total weight of the 10tab is 5278 mg. I take a sample equivalent to 100mg of tablet and I diluted it in 100mL, I took an aliquot of 10mL and I diluted it in 250mL, of these I took 25mL and I diluted them in 250mL. Calculate the weight per tablet. Absorbance = 0.944Acetaminophen Elixir 120 mg/5 mL During the Covid-19 epidemic, the public were advised high fever was a common side effect of infection and to ensure they had acetaminophen on hand. Because of this, there was a sudden nation-wide shortage of commercial liquid acetaminophen. The local Doctor asked if you could compound a mixture for a young child with otitis media as the Mother cannot find any commercially. The Pharmacist did some research and found the following referenced formula for you to use. The Doctor would like to give 50 mL. The child weighs 16 kg. Formulation: Acetaminophen 120 mg 90% ethanol 0.7 mL Propylene glycol 0.5 mL Purified water 0.5 mL Glycerol qs ad 5 mL Dissolve acetaminophen in ethanol and propylene glycol, add most of the glycerol, add water, adjust to volume with glycerol. Strength: 120 mg/5 mL Child dose: 15 mg/kg q4-6h. Maximum 4 doses in 24 hours. Warnings: Contains 13% alcohol EUD: As per local guidelines. Questions: Calculate appropriate…