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Explain why it is important to use only a small amount of bacteria when preparing a smear
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- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. What must be done with the bacterial sample after it is processed in the sterilizer?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
- What is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubeOven sterilization of aqueous culture medium. Effect: Rationale:Why is it best to use sterile distilled water in the preparation of microbial suspension and dry smears for slide preparation? What possible error could happen if a glass slide is reused for slide preparation?
- during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:Kligler’s iron agar and SIM are multiple test media.. Both media are stabbed but for different reasons. Explain.
- Serial dilution is recommended prior to pour and spread plate. Why is this so?In paragraph form (about 2-3 long paragraphs), how would you characterize and identify antimicrobial agents from root samples using chromatography and electrophoresis? Please be in full details.The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a culture dish, thinning a sample and isolating individuals", describes which technique?