For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safranin
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For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).
|
Stain |
Cationic or Anionic Dye |
|
malachite green |
|
|
crystal violet |
|
|
nigrosin |
|
|
safranin |
|
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- What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic acid mycolic acid phenol acid fuschin malachite green safranin nigrosin iodine ethanol methylene blue crystal violet carbol fuschin Congo red acid-alcohol O O O O O O O O O O O O O OExplain the mechanism of staining for each of the stains used. Iodine Methyl green NigrosinMethylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.
- Select all of the following that represent examples of positive stains. Check All That Apply nigrosin methylene blue crystal violet India ink safranin21 22 23 24 Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to create the photo. Make sure to describe the structures indicate by the arrow in letter A and the structures circled in letter B. Is this a simple or differential stain? Explain your answer. 1- OB 10 μmBoth crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.
- Give the color reaction in Wright’s stain and the function of these cells.Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative stainingChoose the dyes or reagents used in the Endospore stain. (More than one choices are correct) a acid alcohol b crystal violet c carbolfuschin d malachite green e safranin f iodine g alcohol h methylene blue