Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid Schiff technique 2. Grocott's hexamine silver technique
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Q: cold method of Acid-fast staining.
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Write the instructions, reagents, principles and the procedures for the following.
1. Periodic acid Schiff technique
2. Grocott's hexamine silver technique
3. Ziehl-Neelsen stain
4. Lactophenol blue stain
5. Mehtamine silver technique
6. Shikata's orcein method
7. Mann's methyl blue-eosin stain
Step by step
Solved in 3 steps
- Complete the table below. For the function, identify the reagent if it is a stain, clearing agent, mountant, fixative, dehydrant, embedding medium, or adhesive. Then give 2 common health hazards of each. Reagent Function Health hazards 1. FAA 2. Ethyl alcohol 3. Xylene 4. Acetone 5. 10% formalin 6. paraffin 7. Harris Hematoxylin 8. Mayer's albumin 9. Canada balsam 10. Distilled waterwrite the protocol for mann's methyl blue-eosin staining, including their reagents, materials, prinicple, procedures results, and cautionsWrite the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.
- Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.Name all the reagents used in Gram-staining and their roles in the staining procedure.
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayAnswer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.Salmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…