For the reaction E + S <> ES, what is the [E] if [S] = 0.18 mM, [ES] = 133 uM, and the Kd = 15 uM? 11,000 UM 20 uM 11 uM 180 uM
Q: Which of the following is the most efficient enzyme An enzyme with a high kcat and a high Km. An…
A: Enzymes play a fundamental role in biological processes by catalyzing chemical reactions, thereby…
Q: The approximate thickness of the phospholipid bilayer is 10A 1 micro M 50A 150A 1nM
A: There are four classes of biological macromolecules - nucleic acid, proteins, lipids and…
Q: Rank the following fatty acids from lowest melting temperature to highest melting temperature. (A)…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons. The…
Q: In the absence of an enzyme, a reaction has a Keq = 10¹5. i. Is AGO for the reaction + or -? ii.…
A: In a general reaction such as:aA + bB ⇌ cC + dDAt equilibrium (steady state), the concentration of…
Q: The reaction coordinate diagram shown below is for the mitochondrial enzyme ATP synthase. G KJ/mol…
A: A reaction coordination graph is a graphical representation of the free energy changes that occur…
Q: is the sum of all chemical reactions in the body. It allows the body to convert energy from oods…
A: Metabolism is a fundamental and intricate biochemical process that underpins the functioning of…
Q: Given the following atomic weights, what is the molecular weight of the following triglyceride if n…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons.…
Q: People are very interested in understanding how prions fold. (Prions are proteins that begin…
A: 1. To determine which protein, mouse or bovine, will be more "folded" at 310K, we need to compare…
Q: Assume a protein is composed of 120 amino acid residues and that each amino acid can have three…
A: Proteins are primarily polypeptide chains. A polypeptide chain is formed by amino acids. Amino acids…
Q: Briefly list out the chemicals contributing the anti-oxidative activities found in green tea.
A: Green tea is the most widely consumed beverage, following water.The chemicals present in green tea…
Q: 2. You made a pH=9.8 buffer solution by mixing NaOH and glycine to give a solution that is 0.20 M in…
A: In order to solve (d), first we need to calculate (c). So, here is a brief and comprehensive…
Q: Which of the following would be a valid symbol identifier of the fatty acid shown below? HO |…
A: There are four classes of biological macromolecules; proteins, nucleic acids, lipids and…
Q: HO-CH2 НО ОН H 0 Н но-сн, Н ОН H ОН Н Н ОН но-CH2 Н Н Н OH Н H OH HO-CH, H ОН H о он ОН H List the…
A: Monosaccharides are connected by glycosidic bonds. Glycosidic bonds are covalent bonds, formed when…
Q: Cold-blooded animals modulate the fatty acid composition of their membranes as a function of…
A: Answer :- Animals classified as cold-blooded, or ectotherms, depend on the outside temperature to…
Q: What is the relationship between lactate production and lactate remov
A: Lactate production and lactate removal are closely related processes in the human body, both of…
Q: 1a. Name and draw diagrammatically the series of mitochondrial electron transfer catalysts, starting…
A: ETC consists of four protein complexes called Complex I, II, III and IV that transport electrons…
Q: Draw a mechanism using the general features of a serine protease to explain how inhibitor x might…
A: Inhibitors can be broadly classified into reversible and irreversible inhibitors. If the bond…
Q: 10.) ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. Fill in the…
A: Amino acids are the building blocks of protein. Some amino acids in the protein undergo…
Q: Case Study: Enzyme Kinetics Data for new statin drug (inhibits HMG CoA reductase): I Substrate (UM)…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: 1a. If you wanted you could take a glucose molecule and convert it to pyruvate via glycolysis and…
A: Hello! Due to time constraints, we are able to provide the answer for the first sub part only. If…
Q: G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs) are two basic receptor…
A: GPCR stands for G Protein-Coupled Receptor a type of cell surface receptor involved in signaling.RTK…
Q: The dominant motif found in hemoglobin and myoglobin is: a) twisted beta sheet b) beta barrel c)…
A: 1)The dominant motif found in hemoglobin and myoglobin is (b) beta barrel.2)myoglobin consists of (d…
Q: An enzyme with concentration of 3.0 uM is added to a flask containing 125 uM of substrate. You know…
A: The observed velocity (Vo) in enzyme kinetics denotes the initial rate of an enzyme-catalyzed…
Q: Determine whether each of the carbohydrates is a monosaccharide, disaccharide, or polysaccharide.
A: Carbohydrates are biomolecules that comprise carbon, hydrogen and oxygen atoms. The hydrogen-oxygen…
Q: Complete the Haworth projection of B-D-fructose. The anomeric carbon is shown. C H Answer Bank OH…
A:
Q: If a cell were given glucose labeled at carbon-3 with 14C, what carbon(s) of pyruvate would contain…
A: Glycolysis is a process by which a cell converts one glucose molecule to 2 molecules of pyruvate.…
Q: Choose the TRUE statement. A phospholipid micelle (not a liposome) can be characterized as Forming…
A: Phospholipids are important components of cell membranes and can form various structures in aqueous…
Q: ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. 7.) Which Two Amino…
A: There are four classes of biological macromolecules: they are proteins, nucleic acids, lipids and…
Q: What is the difference between fast and slow glycoly
A: Fast and slow glycolysis are two distinct metabolic pathways that cells employ to generate ATP…
Q: What is the process of beta oxidation
A: Beta-oxidation is a metabolic process that occurs in the mitochondria of cells and is responsible…
Q: You identify a section of 2 beta strands in a protein crystal structure. When you identify the…
A: The linear arrangement of amino acids in beta strands, an usual secondary structure in proteins,…
Q: Match the descriptions to the type of G protein. Heterotrimeric G proteins monomeric in both active…
A: G proteins (Guanosine nucleotide-binding proteins) can be broadly classified into two types. They…
Q: Why are several glycine residues found within the selectivity filter of K+ channels? a. Glycine…
A: The selectivity filter of potassium channels is the structural element directly responsible for the…
Q: TPCK is a molecule with large hydrophobic groups. Why does TPCK inactivate chymotrypsin but not…
A: Proteases are enzymes responsible for cleaving peptide bonds. Chymotrypsin and trypsin are both…
Q: Draw the chemical mechanism (with electron-pushing arrows) for the addition of an RNA nucleotide…
A: The process by which m RNA is synthesized from DNA is known as transcription. RNA polymerases are…
Q: 10) Explain how the Km and [VO] of a reaction between a fixed amount of catalase and a gradually…
A: An allosteric inhibitor doesn't competes with the substrate for binding at active site. It binds to…
Q: N Signal Sequence The schematic above depicts a protein with the indicated signaling sequences along…
A: Protein sorting is the process where the proteins are transported to their particular destination or…
Q: The scientific name of the fructose molecule shown below is CH2OH OH НО ОН CH₂OH
A: Fructofuranose is a structure which is similar to the furan ring, which has a five carbon membered…
Q: An enzyme catalyzes a reaction with a Km of 6.50 mM and a V of 4.45 mMs. Calculate the reaction…
A: The Michaelis-Menten equation to calculate the reaction velocity of enzymatic catalytic reaction is…
Q: Biktarvy, shown below, is a new drug that is being investigated for the treatment of HIV. но OH F…
A: In biochemistry a functional group is a group of atoms that is responsible for the chemical…
Q: Glycogen can form alpha 1-->4 and beta 1--> 6 linkages alpha 1--> 4 and alpha 1--> 6 linkages alpha…
A: Classification of carbohydrates: Monosaccharide: it composed of single sugar molecule. Example:…
Q: A) Effect of Enzymes on Reactions Which of the following effects (a-g) would increase the rate of an…
A: We know Keq is the equilibrium constant and it is equal to .Here;[P] is the concentration of product…
Q: A protein has binding affinity for its ligand of KA = 2 x 105 1/M at pH 5.0 and room temperature. a.…
A: Kd is the dissociation constant for a protein-ligand complex that measures the affinity with which a…
Q: Dimethoate is a commonly used agrochemical. However, chronic exposure to it can cause an individual…
A: Hyper-ß-alaninemia is a condition characterized by excessive alanine in the muscles. In the context…
Q: Imagine you need to move a large protein from one region in the cell to another. Which of the…
A: Nucleases are enzymes that break down nucleic acids (DNA and RNA) by cleaving the phosphodiester…
Q: Identify factors that account for the high phosphoryl-transfer potential of the nucleoside…
A: High phosphoryl transfer potential is the measure of tendency of a molecule to donate its phosphate…
Q: The most immediate result of ATP hydrolysis for the sodium/potassium ATPase is the... binding of ADP…
A: Na,K-ATPase is a membrane protein which plays a vital role in the cell. It pumps Na+ and K+ ions…
Q: In one type of hemoglobin variant, lysine EF6 is replaced by an aspartic acid residue. What would be…
A: The replacement of lysine (K) by aspartic acid (D) at position EF6 in hemoglobin affects the…
Q: You discover a new polysaccharide that has no branching whatsoever. What do you predict is the role…
A: A polysaccharide is a type of carbohydrate made up of long chains of sugar molecules linked…
Q: Assume a fragment of two-stranded antiparallel β-structure is 3.3 nm long. What is the approximate…
A: Deoxyribonucleic acid also known as DNA, is a molecule that houses the genetic instructions required…
![For the reaction E + S <=> ES, what is the [E] if [S] = 0.18 mM, [ES] = 133 uM, and the Kd = 15
uM?
11,000 UM
20 uM
11 uM
O 180 UM](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa0e64ef2-ef33-42d4-8972-9fbccb33a1ca%2F1ae4101f-9110-4189-a360-909e3cc0aafd%2Fkarpozu_processed.jpeg&w=3840&q=75)
Step by step
Solved in 4 steps
- In your acid phosphatase enzyme kinetics lab, you constructed a Lineweaver-Burk plot. Lefs assume that you graphicaly obtain 1/Vmax - 9.33 (umoliey and -1Km--0.012 M. Which of the following is correct? A) Vmax = 9.33 (no units) B) Km = 0.012 (no units) C) Vmax = (1/Vru" = 0.107 umolis D) Km =-14-1K = 83.3 pM E) Both C) and D) are correctPlease note the following Lineweaver-Burk plot for the enzyme Virbraniumase reacting with a substrate: -0.4 . 1/V 5 4 3 2 -0.2 0 0.2 y = 5.2781x + 1.3338 R² = 0.9967 0.4 0.6 0.8 1/[S] 1 Based on the information provided, what is the Vmax for this reaction?An enzyme has the following values: vmax's; 0.028 M s-' and 0.021 M s-' (I), and km's; 0.00198 M and 0.00197 M (I), when the total concentration of enzyme is 10-º M. The (I) values were determined with 1 microM inhibitor. Select the value closest to the k, for this enzyme. (hint 1 microM = 10-6 M).
- The enzyme serine hydroxymethy ltransferase (SHMT) catalyses the conversion of serine into glycine. The fo llowing table gives the initial rates, vo, for the SHMT-catalysed reaction of the substrate serine at var ious concentratio ns of serine, lSI.[S]/(mmol dm-3) 10 20 30 40vo(μmol dm-3 s-1) 1.63 2.94 4.10 4.95Use the data to determine the values of the MichaelisMenten constant, the maximum velocity of the reaction, and the maximum turnover number of the enzyme.From a kinetics experiment, the Vmax was determined to be 450µM∙min-1. For the kinetic assay, 0.1mL of a 0.05mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 125,000 g/mole. Assume a reaction volume of 700µL. Calculate the kcat (in sec-1) for the enzyme.A Lineweaver-Burk plot generates a line with the following formula: y = 0.3x + 0.4. What is the Km of this enzyme?
- Suppose the biochemical pathway represented in Equation 5-10a is coupled at its first step to the hydrolysis of ATP to ADP. How would this change the expres- sion for AG of the pathway?1. A Lineweaver-Burk Plot is shown below. 30 25 Curve A y = 3.1207x + 2.4978 20 15 Curve B y = 1.0003x + 2.3602 10 5 -3 1 5 7 11 1/[Catechol] (mM1) With these curves, determine the following enzyme parameters. Show all pertinent solutions. a. Km of Curve A and Curve B b. Vmax of Curve A and Curve B c. Assuming that one of these curves corresponds to the kinetics of one enzyme and one substrate, which curve represents the effect of an inhibitor? Why do you say so? d. What type of inhibition is exhibited by your answer in question c? Why do you say so? 1/V, (units of activity 1)If a data from an enzyme experiment is plotted as a Lineweaver - Burk plot, and the Vmax is 0.02 sec/mol, and x-intercept is -2.5 mM-1, then what is the KM value?
- From a kinetics experiment, kcat was determined to be 295sec-1. For the kinetic assay, 0.3mL of a 0.25mg/mL solution of enzyme was used, and the enzyme has a molecular weight of 125,000 g/mole. Assume a reaction volume of 3mL. Calculate Vmax (µM∙min-1) for the enzyme and catalytic efficiency ( in M-1sec-1) for the enzyme. The Km for the enzyme was determined to be 2.55 x 10-2M.Use the concept of Hess' Law to determine AH for the process V 2X +Z given the following numbered processes: V 2 W AH1 W X + Y AH2 Z- 2Y AH3 ΔΗ+ ΔH +ΔΗ3 ΟΔΗ+ 2ΔΗ-ΔΗ3 ΟΔΗ+ 2ΔΗ2+ ΔΗ3 ΔΗ + ΔH,-ΔΗ3What is the likely keq for an enzyme that has a deltaG =-18.3kj/mol?