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In analytical chemistry, when and why do we do representative sampling?
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- Explain the difference between random sampling and haphazard sampling as used in Analytical chemistry.In analytical chemistry, when and why do we do representative sampling? How can you apply this concept in medical laboratory science? *What are the differences between systematic and random errors and how do they effect accuracy and precision? In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample? A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation: y = 44.72x + 4.06 what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?
- PAPER CROMATOGRAPHY • Define chromatography • Demonstrate an understanding of the process of chromatography • Describe the steps involved in a chromatography investigation Explanation Coloured inks are often made up of several different inks. Each colour of ink will get carried a different distance by the water, allowing you to see all of the different inks. Try this with different colours to see what actually goes into a colour. This technique is called chromatography and can be used in chemistry to find out what is in an unknown mixture. II. Materials .Felt tip pens (non-washable) . Kitchen roll .A bowl or cup . Water • Scissors III. Health and safety • Make sure that you thoroughly clean any equipment after the experiment before using for food IV. Procedure Method 1 1. Take a sheet of kitchen roll and draw a simple picture with a non-washable felt tip pen. 2. Place the sheet over an empty bowl. This is to keep the paper from getting too wet. 3. Carefully add a few drops of water…Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.[References) A method for the determination of the corticosteroid methylprednisolone acetate in solutions obtained from pharmaceutical preparations yielded a mean value of 3.7 mg mL with a standard deviation of 0.3 mg mL. For quality control purposes, the relative uncertainty in the concentration should be no more than 3%. How many samples of each batch should be analyzed to ensure that the relative standard deviation does not exceed 9% at the 95% confidence level? samples
- What are the types of gravimetric methods? Give an examples where each method is used in an analysis. What is particulate gravimetry? When do we use this technique?1. Differentiate the following terms. Accuracy Precision 2. What are the advantages of using the analytical balance in analytic chemistry? Enumerate at least two then explain each. 3. What are the factors affecting the discrepancies in accuracy and precision? Enumerate at least four then explain each.What is the purpose of a calibration curve in analytical chemistry?
- One method for quantitative determination of the concentration of constituents in a sample analyzed by gas chromatography is the area normalization method. In this method, complete elution of all of the sample constituents is necessary. The area of each peak is then measured and corrected for differences in detector response to the different eluates. This correction is accomplished by dividing the area by an empirically determined correction factor. The concentration of the analyte is found from the ratio of its corrected area to the total corrected area of all peaks. For a chromatogram containing three peaks, the relative areas were found to be 16.4, 45.2, and 30.2 in the order of increasing retention time. Calculate the percentage of each compound if the relative detector responses were 0.60, 0.78, and 0.88, respectivelyWhat is paper chromatography please include the importance of using itWhat are the advantages and disadvantages of volumetric and gravimetric analysis?