Q: The first step in identifying an organism from a pure working culture is to perform a(n) __________…
A: The pure culture consists of a single microorganism. This culture helps to easily evaluate the…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…
A: Staphylococcus aureus It is a Gram positive coccus bacteria. These bacteria have the very distinct…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
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Q: Describe the Gram staining procedure
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Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…
A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…
Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…
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Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…
Q: If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is…
A: Option b
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
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Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
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Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
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Q: Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please…
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Q: Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the…
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Q: what is gram staining
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Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Stains Primary Stain Mordant Destain Secondary Stain Diagram of Stain Simple Stain N/A N/A N/A Gram…
A: This question is regarding different staining techniques of microorganisms.
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
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Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
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Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…
A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…
Q: Please provide the principle of gram staining method.
A: Introduction: Gram staining is the most widely used differential staining. It is based on the…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: Define the term gram staining.
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Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
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Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…
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Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…
A: answer given below
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: After performing the gram stain you observe your result as gram negative. To help confirm this…
A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…
Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…
A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Gram stain: Cell shape: Based on your above results, indicate which tests need to be done & what…
A: Microbiology is the branch of science which deals with the study of organisms which are too small to…
Q: Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
A: Gram staining is a most important technique in the microbiology to differentiate the group of…
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: What are the expected result after gram staining? a. Crystal Violet = b. Gram Iodine = c.…
A: Gram staining is a type of staining technique used to differentiate bacteria based on their cell…
Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…
A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…
Q: Gram-staining
A: Reagents using in Gram-staining are : Crystal violet Iodine Alcohol/Acetone Safranin
Q: What is the identification of the unknown bacteria
A: Tetrad form are only seen in Gram positive cocci bacterium.
Q: cold method of Acid-fast staining.
A:
Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…
A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…
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- Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingobserve the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the aboveWhat will happen if you FAIL to do the following during bacterial cell staining.a. Smear Preparationb. Heat fixingc. Acid fuchsin d. Steaming of cells with endosporee. Mordant
- The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. SafranineAmong the many methods, please identify Bacillus cereus bacteria, in staining reactiontests, such as A.simple staining B.gram staining C. isolation tests To distinguish specific cultural characteristics of Bacillus cereus bacteria, please explain how to identify Bacillus cereus in these tests below? D indole test, E.starchhydrolysis test F. citrate test.The bacterium Bacillus anthracis was stained with gram staining, endospore staining, acid-stain staining and capsule staining. What result would you expect to see? a. none of these answers b. gram positive, endospore negative, acid-fast negative, capsule positive c. gram negative, endospore positive, acid-fast positive, capsule negative d. gram positive, endospore positive, acid-fast negative, capsule positive e. gram negative, endospore positive, acid-fast negative, capsule negative thank you!!
- Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is not part of the process, put 0. a. Put a loopful of bacteria to the slide. b. Air dry then heat fix the slide. c. Add crystal violet to the slide. d. Add congo red to the slide. e. Apply ethanol.Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary stain:b. Mordant:Lab reportExPEriMEnt9 90 Experiment 9: lab reportc. Decolorizing agent:d. Counterstain:
- the purpose of a mordant in Gram stain is a. to prevent the crystal violet from leaving the cell b. to make bacteria cell larger c. to fix iodine inside the cell d. to remove simple stainWhich of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testThe acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method