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Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used in BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO₂ Absorbance 0.1 0.183 T 0.3 0.542 0.5 0.914 0.7 1.298 1 1.61 In order for this to work, samples must be diluted prior to measurement so that the concentration falls within the range of the standard curve. If a sample underwent a 30 dilution, and produce an absorbance value of 0.953, what is the resultant concentration of NO₂ in mg per L? Report your answer to two decimal places. Tip: The regression equation will give you the final concentration. The dilution is equal to V₁/V₂. The question is asking for the initial concentration.

Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used in BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO₂ Absorbance 0.1 0.183 T 0.3 0.542 0.5 0.914 0.7 1.298 1 1.61 In order for this to work, samples must be diluted prior to measurement so that the concentration falls within the range of the standard curve. If a sample underwent a 30 dilution, and produce an absorbance value of 0.953, what is the resultant concentration of NO₂ in mg per L? Report your answer to two decimal places. Tip: The regression equation will give you the final concentration. The dilution is equal to V₁/V₂. The question is asking for the initial concentration.

Chemistry: Principles and Practice
Chemistry: Principles and Practice
3rd Edition
ISBN:9780534420123
Author:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Publisher:Daniel L. Reger, Scott R. Goode, David W. Ball, Edward Mercer
Chapter14: Chemical Equilibrium
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Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO3 Absorbance 0 0.1 0.3 0.5 0.7 o 0.183 0.542 0.914 1.298) 1.61) In order for this to work, samples must be diluted prior measurement so that the concentration falls within the range of the standard curve. If a sample underwent a 30 dilution, and produce an absorbance value of 0.953, what is the resultant concentration of NO₂ in mg per L? Report your answer to two decimal places. Tip: The regression equation will give you the final concentration. The dilution is equal to V₁/V₂. The question is asking for the initial concentration.
Transcribed Image Text:Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO3 Absorbance 0 0.1 0.3 0.5 0.7 o 0.183 0.542 0.914 1.298) 1.61) In order for this to work, samples must be diluted prior measurement so that the concentration falls within the range of the standard curve. If a sample underwent a 30 dilution, and produce an absorbance value of 0.953, what is the resultant concentration of NO₂ in mg per L? Report your answer to two decimal places. Tip: The regression equation will give you the final concentration. The dilution is equal to V₁/V₂. The question is asking for the initial concentration.
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