Q3. Lacasses are found in plants, insects and bacteria, but the most important sources of these enzymes are basidiomycetes. Pleurotus sp. Was cultivated in Medium 1 for 7 days and then transferred to Medium 2 for the production of laccase under submerged fermentation for 14 days as shown in the following table: Medium 1 Medium 2 Composition g/L 10. 2.0 Composition Dry ground mandarine peels ZnSO,+7H2O g/L 40 0.002 Glucose NHẠNO3 KH2PO4 Na;HPO4•7H;O MgSO4•7H2O Yeast extract Source: Stajić et al. (2006). Enzyme and Microbial Technology, 38:65-73 0.8 0.75 FESO4•7H2O | CaCl;•H2O CUSO7•H2O 0.005 0.06 0.5 0.02 2.0 MNSO4•7H2O_ 0.05 (a) State the function of Medium 1 and Medium 2. (b) Explain the important of Medium 1 in the production of laccase.

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Chapter24: Fungi
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Please answer the following question : (a.) and (b.)

Q3.
Lacasses are found in plants, insects and bacteria, but the most important sources of these
enzymes are basidiomycetes. Pleurotus sp. Was cultivated in Medium 1 for 7 days and
then transferred to Medium 2 for the production of laccase under submerged fermentation
for 14 days as shown in the following table:
Medium 1
Medium 2
Composition
g/L
10.
Composition
Dry ground mandarine peels
ZNSO4+7H2O
g/L
40
Glucose
NH,NO;
2.0
0.002
0.005
0.06
KH2PO4
0.8
FeSO4+7H2O
Na,HPO4-7H2O
0.75
CaCl2•H2O
MgSO4•7H2O
Yeast extract
Source: Stajić et al. (2006). Enzyme and Microbial Technology, 38:65-73
0.5
CUSO7•H2O
0.02
2.0
MNSO4•7H2O
0.05
(a)
State the function of Medium 1 and Medium 2.
(b)
Explain the important of Medium 1 in the production of laccase.
Transcribed Image Text:Q3. Lacasses are found in plants, insects and bacteria, but the most important sources of these enzymes are basidiomycetes. Pleurotus sp. Was cultivated in Medium 1 for 7 days and then transferred to Medium 2 for the production of laccase under submerged fermentation for 14 days as shown in the following table: Medium 1 Medium 2 Composition g/L 10. Composition Dry ground mandarine peels ZNSO4+7H2O g/L 40 Glucose NH,NO; 2.0 0.002 0.005 0.06 KH2PO4 0.8 FeSO4+7H2O Na,HPO4-7H2O 0.75 CaCl2•H2O MgSO4•7H2O Yeast extract Source: Stajić et al. (2006). Enzyme and Microbial Technology, 38:65-73 0.5 CUSO7•H2O 0.02 2.0 MNSO4•7H2O 0.05 (a) State the function of Medium 1 and Medium 2. (b) Explain the important of Medium 1 in the production of laccase.
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