Result nad Discussion Lead Acetate Reaction: Samples: lysine, cysteine, methionine Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2 -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.
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Result nad Discussion
Lead Acetate Reaction:
Samples: lysine, cysteine, methionine
Reagents: 10% Sodium Hydroxide (NaOH) and Lead Acetate Pb(CH3COO)2
-To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (40%) and boil the contents for 5-10 mins over a bunsen burner. Cool the contents and add few drops of 10% Lead acetate solution and observe.
Step by step
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- Results and Discussion: Sakaguchi Reaction: Samples: arginine , glycine, tryptophan Reagents: a-naphthol , Sodium Hypobromite (1ml Bromine water added to 1ml 10% NaOH, freshly prepared) -Mix 1 ml of 10% NaOH with 0.5 ml of the sample and add 1 ml of alpha-naphthol reagent. Then add drop by drop (about 5 drops) of the sodium hypobromite solution. Note the color formed.Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____Table 1 - Comparison of the effect of catechol concentration on the amount of product formed. Absorbance Potato extract Absorbance 0 mins after 30mins (2nd reading) (mL) 1st reading 1 Tube # la blank 2a 3a 4a 1 1 1 dH₂O Catechol (mL) (mL) 7 5 3 1 0 2 4 6 0.00 0.060 0.033 0-05-2 Q4) Give 2 reasons for adding dH₂O to these tubes in Table 1? Time for reading: 3:21 -0.11 Absorbance: Time for reading: 3.36 Q5) Tube la serves as a control, but why is this control needed? Absorbance: 0.197 Time for reading: 3.37 Based on the data from Table 1 answer these questions: Q1) What is the name of the enzyme found in potato extract? Answer: catechol Q2) What is the substrate? Answer: THO Q3) Name of product of this enzyme catalyzed reaction? Answer: Absorbance: 0.152 Time for reading: 3:39 Absorbance: . 166 ness Catechol Benzoquinone Subtract 1st from 2nd reading -0.01 0-137 0.11.19 0.119 Q6) Notice that your 1st absorbance reading in tubes 2a-4a are quite similar but it then becomes very different…
- Penicillin G potassium 5000 units Isotonic Sodium Chloride Solution ad 15 mL Sig. Nose drop Using soluble penicillin tablets, each containing 100,000 units of crystalline penicillin G potassium, explain how you would obtain penicillin G potassium needed in compounding the prescription.Computation: Ratio Strength, PPM, mg%Show your complete solution.1. How many grams of a 1:80 trituration of strychnine sulfate should be used for this prescription?RxStrychnine sulfate 45mgBelladonna extract 360mgSucrose 36gFt. chart; Div. in #XXXVIAmount of Strychnine in grams = ?Results and Discussion: Folin's McCarthy Sullivan Test: Samples: proline, methionine, alanine Reagents: sodium hydroxide (5N), Glycine (1%) and 10% sodium nitroprusside solution, 6N HCl -To 1 ml of the amino acid solution taken in a test tube, add few drops of sodium hydroxide (5N), followed by addition of few drops of glycine (1%) and 10% sodium nitroprusside solution (HANDLE WITH CARE)and vortex. Place the test tube in a hot water bath, maintained at 40 C, for 15 minutes. Cool the test tube in ice cold water for 5 minutes and add 0.5 ml of 6N HCl. Vortex the contents and allow to stant fpr 15 minutes at room temperature.
- Example of a Protein Purification Scheme: Purification of the Enzyme Xanthine Dehydrogenase from a Fungus Volume Total Total Specific Percent Fraction (mL) Protein (mg) Activity Activity Recovery 1. Crude extract 2. Salt precipitate 3. Ion-exchange chromatography |4. Molecular-sieve chromatography 5. Immunoaffinity chromatography 3,800 22,800 2,460 0.108 100 165 2,800 1,190 0.425 48 65 100 720 7.2 29 40 14.5 23 1.8 275 152.108 11 Calculate the specific activity of step#4. Note that percent recovery=% Yield.COLOR REACTION OF PROTEINS Question Guide: 1. Write the positive result, the chemical group responsible for the positive and the importance of each test. Test Positive Result Chemical Group Biuret Ninhydrin Xanthoprotei Millon's Hopkins-Cole Lead Acetate Pauly Bromine Water SakaguchiGive two advantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.
- Alligation Alternate. Show your complete solution. Note: Indicate the unit. Indicate your answer in whole numbers. 1. The solvent for the extraction of a vegetable drug is 75% alcohol. How many milliliters may 95%, 60% and 50% alcohol be mixed to prepare 75% of 4 pints of alcohol? Amount of 95% alcohol needed = ? Amount of 60% alcohol needed = ? Amount of 50% alcohol needed = ?give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.Rx Clindamycin phosphate 4% w/v Propylene glycol 12.9% w/v Purified water 11% w/v Isopropyl alcohol ad 244 mL (Sp.G. = 0.880) Ft. clindamycin gel Sig.: Place thin layer onto affected areas How many grams of isopropyl alcohol are present in a clindamycin gel that has 76 mL of purified water? Assume negligible volume shrinkage as result of mixing these ingredients. Answer: 415.2 (please show all work) ANSWER IS ALREADY GIVEN PLEASE SHOW WORK IN DETAIL HOW IT COMES