The three standards have different retention times on the Mini-GC. What physical characteristics of the molecules do you think contribute to this?
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The three standards have different retention times on the Mini-GC. What physical characteristics of the molecules do you think contribute to this?
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- 1. In thin layer chromatography (TLC), explain how the pigments separate on a piece of paper. 2. If the spots are colorless on a TLC plate, how can you make it visible? Enumerate 2 ways and discuss each.2. Consider the following example GC Chromatogram from a publication about aggression among Mediterranean Acrobat Ants (C. scutellaris). This particular sample was generated by rinsing hydrocarbons from ant exoskeletons. From the chromatogram, what is the minimum number of unique compunds present in the sample? What data led you to that conclusion? (Don't overthink it) 2 8000000 7000000 6000000 5000000 4000000 3000000 2000000 1000000 Counts (Abundance) 5 19 6 7 0 23.00 24.00 10 | 11 13 17 20 14 15 16 12 teel | 22 19 21 ли 25.00 26.00 27.00 Time "Example of a Gas chromatography-Mass spectrography (GS-MS) chromatogram of hydrocarbon molecules in C. scutellaris" by Filippo Frizzi, et.al, in PLoS One is licensed under CC BY 4.0Can paper chromatography be used to separate and identify very volatile substances? Why? Please explain comprehensively.
- Gas Chromatography (GC) is one of the useful techniques in analytical chemistry. Give two important components in GC.Explain the following Chromatography (for drug analysis) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)Why is strict implementation of LQMS (Laboratory Quality Management System) or GLP is necessary ? What are the elements ?
- 19. What is thin layer chromatography (TLC) LEAST useful for from the list below? A) To determine if crystallization or distillation may be more effective for a separation B) To determine the number of components in a mixture C) To determine the appropriate solvent for column- chromatographic separation D) To monitor the progress of an organic synthesis reaction 14. The solubility of salicylic acid in water is 7.8g/100ml at 100°C and 0.25g/100ml at 25°. Estimate how much water is needed to recrystallize a 19g sample of salicylic acid? A) 150ml B) 250ml C) 300ml D) 400mlConsider the following molecules. Which statement(s) is TRUE? CH3 CH3 но ČH3 но ČD3 i. You could differentiate between the two with a spot test. ii. You could differentiate between the two with liquid chromatography. iii. You could differentiate between the two with TLC. iv. You could differentiate between the two with a microcrystalline test. O a. i and iv only O b. ii and iii only O c. iii only O d. i only e. Neither i, ii, iii, nor iv are correct statements.An example of planar chromatography is: Column chromatography HPLC GLC TLC
- Chromatography (DRUG TEST) Thin-layer chromatography (TLC) Gas chromatography (GC) Liquid chromatography (LC) (i.e, high performance liquid chromatography or HPLC)Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?1. How does chromatography separate the components of leave samples? 2. Can you use chromatography to purify a mixture? why? 3. What is Rf value and its significance in chromatography?