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Using 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______ μL
Dilution is the technique of lowering the concentration of a solute in a solution by merely adding more solvents to the solution, such as water. Diluting a solution entails adding more solvent without adding additional solute.
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- In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.Using 25 µl of a patient's serum, how much diluent would be needed to create a 1:2 dilution?
- If the 1-mL sample had been added to 99 mL of diluent to make the first dilution (instead of 9 mL), how many cells would be in that particular dilution?How much serum is needed to create 2μL of a 1:20 dilution?You have a 1:5 dilution. Describe how you would make a 1:100 dilution. When 0.5 µL of serum is added to 1.0 µL of diluent, what is the dilution? What is the dilution factor needed to change a 1:2 dilution to a 1:10?
- A dosage of 10mg is ordered. The solution strength is 2mg in 1 ml. How many ml will be administered? Round to the nearest tenth.You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.The physician has ordered 500,00 units of IV Pfizerpen every 4 hours. How much diluent will you add for a concentration of 500,000 units/mL? How many milliliters will you administer?