Vmax Km x10 TOOLS
Q: How do glucagon and epinephrine affect glycogen in the liver? They increase both glycogen breakdown…
A: Glucagon is a polypeptide hormone secreted by the alpha cells of Iselets of pancreas. Glucagon is…
Q: Construct the two enantiomeric forms/structure of the following monosaccharides and designate the…
A: Monosaccharides are simple sugars, the basic units of carbohydrates. There are two types of…
Q: Given, isas ri Glucose + Pi→ Glucose-6-P ATP + H₂O → ADP + Pi Determine K 'eq for a coupled reaction…
A: Consider a reaction ; A B. Its biochemical equilibrium constant (K'eq 1) will be;Now consider a…
Q: Melting Temperature (Tm) of a dsDNA molecule is the temperature at which a 100% of…
A: The correct answer is:c. 50% of the dsDNA exists in a single-stranded (denatured) form.…
Q: Genetics Question 10
A: The question is asking about the sequence of events that occur during meiosis and the preceding…
Q: What are the donor atoms involved in Aspartame-Cu(II) binding? A) Nitrogen atom of alpha amine group…
A: Aspartame contains two amino acid residues, aspartic acid and phenylalanine, linked by a peptide…
Q: Plot based on chart
A: Standard curve or plot is the graph that is absorbce vs known concentration of proteins. This graph…
Q: Assuming I got 1, 2, and 3 right can you please help with 4?
A: The objective of this question is to determine which generation the phenotype of the F2…
Q: Which of the following hydrogens is most acidic? a A b B с C 11 1 CH₂ A с
A: Answer: b. BExplanation:On removal of the B proton, a carbanion(conjugate base) will form. Higher…
Q: Explain the concept of protein folding and its importance in determining protein structure and…
A: The objective of this question is to understand the concept of protein folding and its significance…
Q: List three important characteristics of plasmids and their function
A: Here is the Three important characteristics of plasmid and their functions.Autonomous…
Q: what is The standard free energy change for the combined system that you wrote in the previous…
A: The standard free energy change (∆G°) of a chemical reaction is the free energy change at the…
Q: 14. A protein mixture consisting of proteins A, B, and C was subjected to various protein…
A: The objective of the question is to determine the properties of proteins A, B, and C based on their…
Q: Draw the reaction between sphingosine and arachidonic acid and draw the full structures
A: Sphigosine is the platform molecule of sphingolipids. Arachindonic acid is a 20 carbon fatty acid…
Q: Luther found a vial containing an unknown lipid sample. He separated the different lipids using gel…
A: The dimensionless number is used in TLC to measure a substance's relative movement concerning the…
Q: 1. From the given DNA strands:a. Identify the template and non-template strand.b. Give the mRNA…
A: The answers are:(a) Template Strand:3' - G T A T A T C A G T T A G A G C T A A T C - 5' Non-template…
Q: What is the relationship between the following monosaccharides (enantiomers, diastereomers, or…
A: Enantiomers are stereoisomers that are mirror images of each other. The D and L forms of a sugar are…
Q: QUESTION 1 You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5,…
A: Proteins fold in a way where the hydrophobic residues are sequestered towards the interior of the…
Q: In N-linked glycoproteins, the sugar molecule is usually bound to a Asp b Ser c Asn d Thr
A: Glycoproteins are conjugated biomolecules where a protein is covalently bound to a carbohydrate…
Q: Question 27 These are all examples of polysaccharides except_ a. starch b. glucose c. cellulose d.…
A: Starch: Starch is a polysaccharide that serves as a storage form of energy in plants. It consists of…
Q: 2. The diagram to the right shows the change in the structure of the C-terminal portion of each of…
A: Therefore, the final answer is: (a) Without the His146 residue, CPA-treated hemoglobin would have a…
Q: Draw A mixed triacylglycerol that contains one saturated 8 - carbon fatty acid chain and two 6 -…
A: Triacylglycerols, or triglycerides, are fats found in living things, storing lots of energy. They're…
Q: 1. Opine dehydrogenases (ODH) have evolved in invertebrate marine organisms with wide-ranging…
A: To determine the L/D configuration of amino acids, place the alpha carbon () in the center, the…
Q: imagine that you are running a sample on a TLC plate that is comprised of aspartate and isoleucine.…
A: The working principle of thin-layer chromatography is the same as column chromatography.A small…
Q: Compare and contrast photosynthesis with oxidative phosphorylation.
A: Photosynthesis is the process by which green plants and algae synthesize sugars using the energy…
Q: STEM Workplace Practices Q6
A: The objective of the question is to determine whether the optimization of an analytical method is…
Q: 8. Draw the product(s) for the following biosynthetic reactions catalyzed by enzyme listed below the…
A: 1.2.3.4.5.
Q: Part 1. In dilute acid, hexoses exist in equilibrium with the corresponding 1,6-anhydrosugars. For…
A: One or more water molecules have been removed from sugars, which are known as hydro sugars. One…
Q: Describe the mechanism behind the hydrophobic effect. Support your description with one drawing.
A: The hydrophobic effect is the tendency of nonpolar molecules to aggregate in aqueous solutions. This…
Q: During the early stages of an enzyme purification protocol, when cells have been lysed but cytosolic…
A: If a reaction velocity v/s substrate concentration curve shifts to the right, then the curve becomes…
Q: Genetics Question 7
A: The objective of the question is to identify the correct event that occurs during the prophase of…
Q: Describe how chymotrypsin is activated via a cascade mechanism in the gut. How does it incorporate a…
A: Chymotrypsin is one of the enzyme present in the digestive juices and it helps in the breakdown of…
Q: Do Km and Vmax get affected by available enzyme concentration? Explain.
A: Enzymes are proteins that catalyse biochemical reactions. Michaelis Menten postulated that free…
Q: In the alpha helix shown below, all carbonyl groups are pointing_ amino groups are pointing…
A: The secondary structure of a protein refers to the folded configuration of the polypeptide chain.…
Q: SARS-CoV-2 Spike protein variant Wuhan Alpha Beta Delta Omicron KD, nM 60 9.6 2.4 1.2 2.4 Using the…
A: Consider a protein 'P' that can bind with 5 different ligands; L1, L2, L3 , L4 and L5.The…
Q: what happens in a normal patient when they consume a large amount of glucose?
A: Glucose is the primary fuel for a living cell. Cellular respiration (glycolysis + citric acid cycle…
Q: what metabolic pathways are more likely to be active during: a) fasting, b) overnight fast) and c)…
A: Intermediary metabolism, also known as metabolic pathways, is the intricate network of chemical…
Q: Tryphtophan is an essential amino acid that is used in the biosynthesis of proteins. Its molecular…
A: Amino acids are biomolecules that have a hydrogen atom, a carboxyl group, an amino group and a…
Q: Question 12 In the tertiary structure of a protein, the R-group of valine can interact with the…
A: Proteins are bio molecules with vast diversity in their structure. They exhibit four levels of…
Q: Identify the major and minor grooves in the DNA molecule PDB ID 141D. In addition, one end of a…
A: The DNA double helix has alternating major and minor grooves. The major groove is wider than the…
Q: Which of the following statements is most accurate ? All statements are accurate All prescribed…
A: The objective of the question is to identify the most accurate statement among the given options…
Q: Name the protein structural element shown in yellow in the figure:
A: Proteins can be defined as large biomolecules. They comprise of one or more chains of amino acids.…
Q: (5) s) Consider the equation for enzyme action below: E-S E-P E+S complex E+P complex Briefly…
A: Catalyst are chemical compounds that speed up chemical reactions but do not undergo any changes…
Q: Draw the structure of cholesterol and write the biosynthetic relations of steroid hormones starting…
A: Cholesterol, a 27-carbon molecule, is the most common steroid in the body and plays a crucial role…
Q: Question 9
A: The question is asking us to identify the enzyme that is responsible for separating DNA strands…
Q: Select the hemoglobin variant(s) that are described by each statement. a) The variant least likely…
A: Haemoglobin is a subunit protein that binds to oxygen and supplies oxygen to the different parts of…
Q: When 10 micrograms of an enzyme with a molecular mass of 80,000 Daultons (grams/mol) is added to a…
A: Before going into the calculations for finding the turnover number (kcat), we need to convert…
Q: bond (s) with the.. 7th and 9th amino acids in the sequence. 12th amino acid in the sequence only.…
A: In an alpha helix, each amino acid residue is hydrogen bonded to the amino acid residue four…
Q: You have expressed a protein of interest in E. coli cells for further study in the lab. The protein…
A: One possible purification scheme for the protein of interest might include the following steps:1.…
Q: Genetics Question 20
A: The objective of this question is to determine the correct order of genes on a chromosome map based…
Step by step
Solved in 4 steps with 1 images
- For the following aspartase reaction in the presence of the inhibitor hydroxymethylaspartate, determine Km and whether the inhibition is competitive or noncompetitive. You have to plot thegraph on the graph paper and also by using excel.[S] V, No Inhibitor V, Inhibitor Present(molarity) (arbitrary units) (same arbitrary units) 1 x 10-4 0.026 0.0105 x 10-4 0.092 0.0401.5 x 10-3 0.136 0.0862.5 x 10-3 0.150 0.1205 x 10-3 0.165 0.142Proline racemase catalyzes the conversion between L-proline and D-proline. The Km and kcat for this reaction are 0.15 M and 550/sec respectively. If the enzyme concentration is 1.45 X 10-5 mmole/ml what is the Vmax of this reaction?The equation of the double reciprocal plot is y = 0.5294 x + 1.4960. What is the value of vmax (in M/s)? The substrate concentration is given in units of molarity (M) and reaction velocity has units of molarity per second (M/s). (Report to three significant figures)
- Calculate AG for the below balanced redox reaction as written. You may find a list of standard reduction potentials useful. 3 12 (s) + 2 Al (s) → 61 (aq) + 2 Al3+ (aq) O-4.24 x 102 kJ/mol O-6.48 x 102 kJ/mol O -2.12 x 102 kJ/mol O-1.27 x 103 kJ/mol O-6.37 x 102 kJ/molexplain in quantitative terms the circumstances under which the following reaction can porceed; L-malate + NAD+ (forward arrow) oxaloacetate + NADH + H+ delta G' standard = +29.7 KJ/molYou make reaction progress curve by plotting absorbance vs time (seconds) and find the equation of the line to be y = -0.00235x + 0.7129. Calculate the U/µL and U/mL of lactate dehydrogenase activity in this fraction. The LDH activity is done identical to what is indicated in the lab manual. Show each step of the calculation from AU/time to M/min, to mol/min, to µmol/min to µmol/min/µL (=U/µL).
- The redox half reaction Oxaloacetate + 2H+ + 2e− → Malate has a standard reduction potential value of 0.166 V. Calculate the free energy (ΔG°′) for the following reaction: Malate + NAD+ → Oxaloacetate + NADH + H+Suppose that the data shown in the margin are obtained for an enzyme -catalyzed reaction. [S] (mM) V (mmol/ml min) 0.1 3.33 0.2 5.0 0.5 7.14 0.8 8.00 1.0 8.33 2.0 9.09 a.) Determine the Km and Vmaxb.) Assuming htat the enzyme present in the system had a concentration of 10-6M, calculate the turn -over numberAcetyl CoA + 2H* + 2e = pyruvate + COASH E = -0.48 V Ubiquinone + 2H* + 2e = Ubiquinol E" = +0.04 V Consider the redox rxn wherein a pair of e passes from pyruvate to ubiquinone. Calculate the change in standard Gibbs free energy (kJ/mol). Report answer to two decimal places.
- The following data were obtained for a reaction that catalyzed by an enzyme: Initial concentration of 0.141 0.109 0.077 0.040 0.028 0.020 0.016 the enzyme [S], mol L- Relative velocity, V 22.0 20.5 19.0 12.5 9.0 7.0 6.0 For this reaction, the rate is found experimentally to follow the Michaelis-Menten equation: Vmax[S] Vo = Км+[S] (1) Transform the above equation into a straight-line equation. By plotting a suitable graph, determine the Michaelis constant (KM) and the maximal velocity (Vmax) for the above reaction. Determine the Vmax value when [S] = KM.What is the actual change in free energy at 37°C for the phosphoglycerate mutase reaction converting 3-PGA to 2-PGA in erythrocytes if after drinking a glass of orange juice the [3-PGA] = 8 mM and the [2-PGA] = 0.08 mM? The standard change in free energy for the reaction is +4.5 kJ/mol. +16.3 kJ/mol O-6.5 kJ/mol O-7.3 kJ/mol -16.3 kJ/mol +7.3 kJ/molCalculate the standard free energy change (AG) for the transfer of electrons from succinate to ubiquinone: Succinate + ubiquinone O-2.7 kJ/mol O2.7 kJ/mol O-8.7 kJ/mol O 14.7 kJ/mol succinate dehydrogenase relevant half-reactions and standard reduction potentials: fumarate + 2 H* + 2 e succinate ubiquinone + 2H + 2 e -6.0 kJ/mol 11 Fumarate + ubiquinol ubiquinol E = 0.031 V E = 0.045 V