Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: What particular features of fluorescence make it useful for microscopy?
A: Step 1 Microscopes are an instrument which is used for observing small objects and finer internal…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: In the differential staining method, various stains are applied. These methods can be utilized for…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: What are the main characteristics of scanning electron microscopy?
A: Electrons are the negatively charged particles having minus 1 charge and represented by e- ,…
Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…
A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: Why are basic dyes more effective for bacterial staining than acidic dyes? State two ways that can…
A: According to the guidelines we have to answer first 2-3 question rest you can ask separately thank…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: What are two things that can be done to improve contrast on a microscope?
A: Contrast refers to the darkness of the background relative to the specimen.
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Which step of the gram stain procedure is most critical?why?
A: Gram staining is one of the most common laboratories test to identify the different bacterial…
Q: What is the purpose of the steam in spore stain?
A: Small tiny objects which are not viewed by the naked eye are called microbes. The microbes are seen…
Q: How does viability staining differ from stains like DAPI?
A: Staining is the technique in biology, which increases the visibility at the microscopic level. Dyes…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Classify H and E staining?
A: Hematoxylin and eosin staining (H and E staining) is a widely used histological staining method used…
Q: What is the advantage of phase-contrast microscopyover bright-field microscopy?
A: Phase-contrast microscopy is an optical microscopy technique that converts phase shifts in light…
Q: What is the secondary stain in the spore stain?
A: Endospores are the structures that are formed by bacteria during unfavorable conditions (lack of…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: What is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain,…
A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…
Q: What is an electron micrograph? Why do electron micrographshave greater resolution than light…
A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…
Q: What major advantage does phase-contrast microscopy haveover staining?
A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: What types of compounds make up the gels used in electrophoresis?
A: The types of macromolecules usually separated on Agarose gel are : Nucleic acids-mainly DNA…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
Q: What is the basic purpose of the paper chromatography?
A: Chromatography is an important analytical method used to separate a mixture of chemical substances…
Q: What is the function of staining in light microscopy?
A: The light microscopy or light microscope represents a laboratory instrument required for the…
Q: How do stains used for light microscopy compare withthose used for electron microscopy?
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
What is a crystal violet stain and its purpose?
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