Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: In the differential staining method, various stains are applied. These methods can be utilized for…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: hich Is The More Important Step In Gram Stain?
A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…
Q: What might happen if the Gram staining procedure is performed ona culture incubated for a little…
A: Gram staining is one of the staining methods used in laboratories to differentiate between two broad…
Q: Is the flagellar stain a Positive or Negative Simple Stain protocol?
A: Staining is a technique that is used to enhance contrast in samples, generally at the microscopic…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: shape
A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…
Q: Are the structural differences between the cell walls of Gram-positive and Gram-negative bacteria…
A: The gram-positive and negative cells differ in their cell wall composition.
Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…
A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: why does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.
A: Acid-fast staining technique is a differential staining technique. The bacteria which get stained by…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: What color will a gram-negative cell be after Gram staining bythe conventional method?
A: Danish bacteriologist Hans Christian Gram developed the Gram staining method. This staining…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Could the Gram stain be used to diagnose the fl u? Why or why not?
A: Gram staining : It is a technique used for differentiation between two large group of bacteria based…
Q: Why is it important to follow sterile technique?
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the…
A: The process of Gram Staining on any sample requires four basic steps that include applying a primary…
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining is performed on the basis of differential characteristics of the cell wall in…
Q: Explain the mechanism of Gram staining.
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining helps in differentiation between gram positive and gram negative bacteria. Procedure…
Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
A: How do Blood agar & EMB agar help us to identify organisms more specifically than a gram stain?
Q: Define the term gram staining.
A: Bacteria are a diverse group of microorganisms with a wide range of shapes, sizes, structures, and…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: List the step-by-step procedure of Gram staining.
A: Gram staining, established by Danish Microbiologist Hans Christian Gram in 1884, is by far the most…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: Why do we need to stain microorganisms?
A: Microbiology is the study of microbes. Microorganisms include bacteria, fungus, archaea, and…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: What is Gram staining?Describe step by step the procedure.
A: GRAM STAINING METHOD:- This is a differential staining that was developed by Dr. Hans Christian Gram…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: What advantage does the oil immersion objective have over the low power objective when doing…
A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…
Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
A: Introduction We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…
A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
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