Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…
Q: Why is it important to limit the quantity of cells used to prepare a smear?
A: A thin layer of cells or tissues that are taken from the body of an organism under study which is…
Q: Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
A: Gram staining is a method in which cells stain either pink (gram-negative) or purple (gram-positive)…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Give two reasons for heating the slide after the smear is air dried?
A: Smear is the thin film of the sample that is spread over the slide to be observed in the microscope.…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: What are Romanowsky stains? Name four of these.
A: Staining is a technique used to enhance contrast in samples , generally at the microscopic level.…
Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…
A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…
Q: Please discuss why staining is important in visualizing microbes under the microscope. What…
A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: Differentiate between negative and positive staining, givingexamples.
A: These are the staining methods that are used to see bacteria under a microscope.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What will happen if you heat fix ypur smear for flagella staining?
A: Falgella staining Flagella is long tail like structure present in most of the bacteria. Flagella…
Q: What are the principle and basic concepts of NEGATIVE staining?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: What is the significance of heat fixing the smear?
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Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…
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Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: What are the functions of a primary stain and a counterstain?
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Q: What are the advantages of UV disinfection versus, or as acomplement to, chemical disinfection with…
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Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
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Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Spores are cellular structures produced by plants, bacteria, and fungi. Spores can be produced for a…
Q: With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation? What…
A: Gram stain Gram staining is a method of staining which is used to classify the bacterial species…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: Why is mordant necessary for the staining of bacterial flagella? Answer in not more than 3…
A: In contrary to negative staining, which leaves the bacteria unstained against a dark backdrop,…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
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Q: Step-by-step smear preparation of Grams stain
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Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: For preparation of a smear on a slide, what is the purpose of heat fixation? What problems can arise…
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Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?
A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…
Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…
A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
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A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: Write down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin…
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Q: Aside from safranin, what other stains can we use for the microscopic examination of onion cells?
A: We can not see all the part of a cell by naked eye. With the help of different types of microscope…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: Describe the procedure for performing a Gram stain. What is the procedure? How does each component…
A: * Gram staining is used to test that whether f bacteria is positive or negative and also be used to…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
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Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: What is the effect when xylene is not removed when staining? What is/ are the specific action/s of…
A: Staining is a procedure in which the coloured dye or a stain is added to a specimen so that it may…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
What is the usual stain used for staining a sputum smear?
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with purple color. Describe the organism with pinkish red color.Why are basic dyes more effective for bacterial staining than acidic dyes?
- In preparing a bacterial smear for staining, heat fixation is done after the smear dries up.a.) Give the purpose of heat fixation.b.) What can be observed in wet mounts or hanging-drop slides that cannot be observed in heat-fixed slides?What are the principle and basic concepts of NEGATIVE staining? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin