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- What is the highlighted structure? BlueRelative fluorescence at 603 nm 1.0 0.8 0.6 0.4 0.2 0.0 L 0.00 0.10 0.20 0.30 Time (s) 0.40 0.50 0.60 In this FRET data table, an antibiotic was added, but the type was not recorded. According to the results, could the buffer being used be either negamycin, tetracycline, rifamycin, or cycloheximide? Explain why based on the FRET data.Identify the image shown in the picture 88 te 林
- 3.1 Given these images focused under different objectives, calculate the size of an onion cell and a waterweed cell. Show your calculations. TREVISIONMIO viewed in HPO A. onion Size: Size: Calculation: mm um viewed in LPO B. waterweed Size: Size: Calculation: _mm um16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X(a) 1 Normalized fluorescence 0.8 0.6 0.4 0.2 0 50 55 OM 0.100 M 0.200 M 0.300 M 0.500 M 1.00 M 2.00 M 60 113588 65 Temp. (°C) 70 75 80 Where is fully folded protein? • Where is fully unfolded protein? • Where is partially folded protein? • To what does SYPRO orange bind? • Why does fluorescence increase as a function of temperature? ● Define a melting temperature for a protein. • Demonstrate how an estimated melting temperature of the protein in zero molar ligand can be determined. • What is the effect of increasing the molar concentration on melting temperature for this protein? • Why is melting temperature a useful measurement to make for a protein especially if you are interested in protein aggregation?
- Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here.Horizontal sequence :VIRL Vertical sequence:MKF Scoring rules: g/o = -3, g/e = -1, match or mismatch - from PAM250 substitution matrix below. SW algorithm. 1. Complete the scoring matrix. Scoring matrix with PAM250 scores: V I R L M K F 2. Set up, initialize and complete the SW matrix. 3. Retrace, align and score alignment(s). Use the arrows and circles for the matrix and path(s). V I R L M K F Align and score all optimal alignments here. PLZ the arrows and circles for the matrix and path(s) AND SHOW ALL possible Alignment21 22 23 24 Short Essay: Answer the prompt in 4 sentances or less. Describe the staining technique used to create the photo. Make sure to describe the structures indicate by the arrow in letter A and the structures circled in letter B. Is this a simple or differential stain? Explain your answer. 1- OB 10 μm
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.(b) Table Q1 below provides optical measurements of samples with known concentrations of analyte (ten samples were measured and the average and standard deviation are presented). They are also represented graphically in Figure Q1, below the table. Analyte concentration (nM) 0.1 0.2 0.5 1 2 5 10 15 fluorescence intensity 300000 250000 200000 150000 100000 50000 0 0 Table Q1 Fluorescence intensity (average) 9300 9200 10800 19600 37200 90000 178000 224000 5 [Analyte]/nM 10 Standard deviation 2000 1200 1000 2000 10000 30000 25000 40000 15 Figure Q1. Signal intensity for different analyte concentrations. Error bars are standard deviation. (ii) Calculate the limit of detection (LOD) for this sensor. (iii) A patient sample measured using this biosensor in the diagnostic laboratory generates a signal of 142800. The coefficient of variation of the device is 5%. Explain whether the diagnostic lab would have the confidence to report that the sample has a concentration in analyte of 8 nM or…paper chromatography: 1.If the Rf of blue was 0.81, Rf of yellow was 0.68, and Rf of red was 0.64, then which color would be the heaviest? which one would be the lightest? and why? Gel electrophoresis: 2.Given that well 1 represented protein from a dog (Rf=0.45), well 2 from a wolf (Rf=0.73) and well 3 from a fox (Rf=0.76), and the unknown well represented protein from a "chupacabra". what animal would say the "chupacabra" is closely related to? 3.which one would be the heaviest and lightest in given above electrophoresis? and why?