Q: Explain how to correctly use the microscope using oil immersion.
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Q: How do you make liquid bacteria culture?
A: It is important for growing more numbers of bacteria to isolate enough plasmid DNA for experimental…
Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…
A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…
Q: layer
A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…
Q: Describe the Gram staining procedure
A: Gram staining is used to differentiate between Gram positive and Gram negative bacteria. These…
Q: What is agar and why is it necessary for growing bacteria on plates?
A: Agar is a mixture of two polysaccharides- agarose and agaropectin. It is derived from red-purple…
Q: Write an introduction related to culture media and isolation of pure culture technique (streak plate…
A: In microbiology, there are five I’s which represent five different techniques used in the…
Q: Why is there a need to use a staining agent in observing the specimen?
A: Staining agent: The things that owes-out stains were recognized as staining agent,…
Q: tate the apparatus used view small samples
A: The world is full of organisms. Organisms have developed from minute single celled structures to…
Q: Sample diagram for the gram staining procedure and microscopic observation.
A: Danish Bacteriologist Hans Christian Gram in 1884 introduced differential staining techniques in…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…
A: Magnification and resolution are important because the magnification in larges the image and the…
Q: Name all the reagents used in Gram-staining and their roles in the staining procedure.
A: Staining is a strategy used to improve contrast in examples, for the most part at the minuscule…
Q: In gram staining, if bacteria retain the primary stain after decolorizing agent treatment. Then the…
A: Answer: GRAM STAINING = It is the differential staining method to differentiate between gram…
Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…
A: Staining is the method of producing different colours that obtained by the reaction of reagents with…
Q: temperature and light source affect the number and type of colonies that can be found in the…
A: Microbes such as bacteria, fungi, viruses are grown at the earth's surface. The atmosphere provides…
Q: How is the correct way of incubating culture plate media? Explain why is that so.
A: After the way of life medium is set, and streaked with the necessary microbe/stock, the cover is put…
Q: Gram staining techniques
A:
Q: Why does fixation of the positive dye help bacteria stick to the slides?
A: Dyes in microbiology are for staining cells; they are used based on the chemical properties and the…
Q: Types of culture media
A: Culture media is a growth medium used in microbiology and biotechnology to grow microorganisms in…
Q: When labeling culture tubes or plates why do we use a pencil instead of a pen? Are there other…
A: Appropriately labeling specimens for research labs is of critical importance.
Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium
A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…
Q: The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin…
A: Electron microscope: It is a microscope which obtains a high-resolution image of non-biological as…
Q: When using a light microscope, objective lenses can be found to have a magnification of all of the…
A: Light microscopes are devices that are used to magnify small objects by using visible light.…
Q: What is the main advantage of using the slide culture technique for identifying molds?
A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…
Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…
A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…
Q: List the reagents of the Gram stain technique in order and their general role in the staining…
A: Gram Stain is used to differentiate the gram positive and gram negative bacteria. The gram positive…
Q: Based on the photo, describe the bacteria observed from the different staining technique. -…
A: Microbiology was a blessing in the eradication of mass-destructive epidemics such as rabies,…
Q: The acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…
A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…
Q: Distinguish batch culture and continuous culture
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Q: If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what…
A: Magnification is defined as a measure of the ability of a lens or other optical instruments to…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…
A: After a clinical sample is received in the laboratory, the first step often is to do a mandatory…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…
A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: What advantage does the oil immersion objective have over the low power objective when doing…
A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…
Q: Why should your solvent be evaporated from the sample placed on the sterile disk before you place it…
A: The antimicrobial activity of natural compounds like essential oils derived from plants can tested…
Q: discuss the microscopy technique that gives the best contrast of nonbiological samples
A: Microscope is used those objects which are not visible under naked eye it is able to view the finer…
Q: The stereoscopic microscope offers a large____________between the objective lens and the specimen.
A: A stereo microscope is utilized for low-magnification applications, permitting superior grade, 3D…
Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?
A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: Briefly, in your own words, describe the steps of the gram staining procedure.
A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
A: The human eye can see objects upto 0.1 millimeters. If the objects are smaller than this, the human…
Q: List the steps of the gram staining procedure in proper sequence and indicate the color of…
A: Gram staining is a method of staining used to distinguish and classify bacterial species into two…
Q: What is this media? What type of media is it? Which type of organism can grow on this plate?…
A: Media is... Agar This is the solids type of Media. Organism type... Streak plate technique is used…
Why sometimes specimen may show organisms under the microscope but not appear in the culture media?
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- Why is it sometimes difficult to attain both good resolution and good contrast at the same time for a sample that is being observed on the microscope stage? Please give a thorough response,Why bacteria require staining before microscopic examination?What advantage does the oil immersion objective have over the low power objective when doing bacterial examination?
- If you were working with an unlabeled simple stained smear, would you be able to identify the bacterial species by observing the slide under the microscope? Why or why not?After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixedYou have a patient sample and have done a Gram Stain. When you look on the microscope, this is what you see. Which of the following tests would you use next? 中 МАС O HE • EMB O MSA
- Why must young cultures be used when doing a Gram stain?You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.Why would specimens viewed with a compound microscope be thin and/or chemically cleared?
- For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainBriefly, in your own words, describe the steps of the gram staining procedure.
