Why is there a need to use a staining agent in observing the specimen?

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: is it essential that the primary stain and counterstain be of contrasting colors

A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…

Q: The thickness of a specimen in view is known as the _______________.

A: Depth of focus.

Q: shape

A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…

Q: describe the agar-overlay technique

A: Biological techniques are processes or methods for trying to study living things. They include…

Q: Why is it important to use a sterilized loop between streaks when preparinga streak­plate?

A: Streaking is a technique used to isolate a pure strain from a single species of microorganism, often…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: What is the significance of properly labeling collected specimen?

A: The specimen collected should be labelled properly before bringing it to the laboratory for testing.…

Q: If you conducted an acid-fast stain on a sputum sample from a person with tuberculosis, what would…

A: The acid-fast staining technique is a differential staining technique. The bacteria which get…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Gram staining techniques

A:

Q: What are the possible sources of contamination that may result during the streak plate procedure?

A: The streak plate procedure is used to isolate microbial colonies in pure form. During the procedure,…

Q: Why is it necessary to use immersion oil when the 100X objective is used'

A: Note - we answer one question at a time. Immersion oil is transparent and has specific viscosity and…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: If you observe a specimen under this 20X objective and the specimen spans one-quarter of the field…

A: A light microscope is common equipment in most laboratories. It comes with one or two eyepieces…

Q: List 3 species of bacteria that can spoil milk and indicate how they react to the gram staining…

A: Spoilage of milk and milk products results from growth of fermentative bacteria when storage…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: Step-by-step smear preparation of Grams stain

A: Gram staining is a laboratory technique that is used to culture bacteria and identifies two kinds of…

Q: Dropping Mercury Electrode

A:

Q: name 5 things about method staining

A: INTRODUCTION Staining Staining is a method that is used in medical field and histology to see things…

Q: What is the main advantage of using the slide culture technique for identifying molds?

A: Side Culture - It is a rapid method of preparing fungal colonies for examination and identification.…

Q: Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure?

A: Plate counting is one of the most fundamental techniques used in microbiology. It is used to…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Q: The acid fast technique is also known as:  A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C.…

A: Acid fast bacteria are gram positive bacteria . They are unique among prokaryotes and normal gram…

Q: list the stains and decolorizing agent used in acid fast staining method.

A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…

Q: List the primary stain, secondary stain, mordant, and decolorizer for the gram stair and write them…

A: The strains in the plate is give on two strains the primary strain and secondary strain. The primary…

Q: Positive Staining Negative staining

A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…

Q: If a specimen is being viewed under a 40x objective, and the microscope has a 10x ocular lens, what…

A: Magnification is defined as a measure of the ability of a lens or other optical instruments to…

Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…

A: After a clinical sample is received in the laboratory, the first step often is to do a mandatory…

Q: The most commonly employed chemical test to determine the presence of blood in stain found at the…

A: Answer is option E"Teichman's test". this test is also called by names haematin crystal acid test or…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: Why do you use oil on the slide to be examined with the oil-immersion objective?

A: Oil immersion microscopy increases the refractive index of a specimen when used in the correct way.…

Q: what are the reagents for the four major steps of the gram stain procedure

A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…

Q: discuss the microscopy technique that gives the best contrast of nonbiological samples

A: Microscope is used those objects which are not visible under naked eye it is able to view the finer…

Q: Draw one acceptable pattern that is used in streak plate method

A: Streak plate method : It is used for the isolation into a pure culture of the organisms , f9om a…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: Why sometimes specimen may show organisms under the microscope but not appear in the culture media?

A: Introduction  We are surrounded by various pathogens such as bacteria, viruses, fungus etc. Bacteria…

Q: What is the hazard of the spattering tendency in heating

A: Sterilization is the process of cleaning a substance by removing, killing or deactivating all forms…

Q: A staining procedure in which one reagent is used and the bacteria takes up the stain while the…

A: Staining is a technique used to enhance contrast in the sample generally at microscopic level. There…

Q: Why should freshly collected samples be used for microscopic examination?

A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…

Q: differential staining procedures

A: Differential staining :  Differential staining is one of the important staining method. In this…

Q: How is staining techniques useful in the clinical settings

A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…

Q: Briefly, in your own words, describe the steps of the gram staining procedure.

A: The bacterial kingdom is classified into Gram positive and Gram negative bacteria depending on their…

Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…

A: Microscopic samples are generally stained prior to analysis as it enhances their contrast and aids…

Q: Describe the use of microscopy to identify microorganisms in patient specimens

A: A microorganism, often known as a microbe, is a minute living entity. Microbiology is the study of…

Q: What is the purpose of examining your specimen slide using low power objective first nefore high…

A: Objective lens are magnifying lens that functions to magnify an image. These lens make an important…

Q: cold method of Acid-fast staining.

A:

Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…

A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…

Q: What staining method would you use to verify that the isolate is not producing survival structures…

A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…

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