You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa -100 kDa -80 kDa 80 kDa -40 kDa 40 kDa a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? (S c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
You observe the following: Your Your friend's Gel Gel 120 kDa 100 kDa 120 kDa -100 kDa -80 kDa 80 kDa -40 kDa 40 kDa a. You and your friend analyzed the same protein sample in your gels. Why are your results different? Explain briefly which gel is the correct representation of the SDS-PAGE & Western blot, and why? b. Explain briefly the size of protein observed in the chromatography column and the Western blot. Are they different or the same, why? (S c. If the primary antibody to Protein A was made in a mouse, describe the details of the secondary antibody used in the Western blot analysis?
Human Heredity: Principles and Issues (MindTap Course List)
11th Edition
ISBN:9781305251052
Author:Michael Cummings
Publisher:Michael Cummings
Chapter13: An Introduction To Genetic Technology
Section: Chapter Questions
Problem 20QP: Analyzing Cloned Sequences A base change (A to T) is the mutational event that created the mutant...
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