Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them. a. Find the done with the gene for lactase. b. Insert the plasmids into bacteria and grow the bacteria into clones. c. Isolate the gene for lactase. d. Create recombinant plasmids, including one that carries the gene for lactase.

Question

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Answer 1

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Students have asked these similar questions

In gene cloning, a vector is required to transform the gene of interest into host cell. State the THREE (3) important regions of the plasmid. Elaborate your answer.

In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).

Using a flow diagram, elaborate on how you would generate a recombinant plasmid.

Answer 2

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Answer 3

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 4

Textbook Question

Chapter 12, Problem 1SQ

Suppose you wish to create a large batch of the protein lactase using recombinant DNA. Place the following steps in the order you would have to perform them.

a. Find the done with the gene for lactase.
b. Insert the plasmids into bacteria and grow the bacteria into clones.
c. Isolate the gene for lactase.
d. Create recombinant plasmids, including one that carries the gene for lactase.

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

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Subscribe now to access step-by-step solutions to millions of textbook problems written by subject matter experts!

Answer 5

Textbook Question

Answer 6

Textbook Question

Answer 7

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 8

Expert Solution & Answer

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 9

Expert Solution & Answer

Answer 10

Expert Solution & Answer

Answer 11

Summary Introduction

To determine:

The sequence of steps that will performed by the individual if he wants to create a large batch of protein lactase using recombinant DNA.

Introduction:

Recombinant DNA technology involves excising the DNA of interest from one genome and inserting into a foreign genome by identification, isolation, manipulation and re-expression of genes.

Answer 12

Answer to Problem 1SQ

Correct answer:

The order of the steps for producing large batch of the protein lactase using recombinant DNA are c.,d.,b. and a.;

c. isolate the gene for lactase,

d. create recombinant plasmid, including one that carries the gene for lactase.

b. insert the plasmids into bacteria and grow the bacteria into clones,

a. find the clone with the gene of lactose.

Answer 13

Explanation of Solution

Recombinant DNA technology is a process that includes transfer of a desired DNA sequence or genes from one organism to a self-replicating genetic element such as bacterial plasmid followed by propagation in host cell. The steps involves in the formation of recombinant are as follows;

The gene of interest is identified and isolated and inserted into plasmid (vector). The joining of DNA from two different sources results in the formation of recombinant DNA plasmid.
The recombinant plasmid is inserted into the bacteria for multiplication. Under the right condition, the bacteria take up the recombinant plasmid.
Each bacterium carrying the recombinant plasmid is allowed to reproduce through the cell division to form the clones of cells.
The identified transgenic bacteria with the gene of interest can be grown in tanks to produce a large amount of desired protein.

Answer 14

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