Chapter 13.3, Problem 25AQP

a.

Summary Introduction

To determine:

The effect of a mutation in an E. coli gene at the $-8$ locations.

Introduction:

Transcription is the basic step of gene expression. In this process, a particular DNA segment with the help of enzyme RNA polymerase forms RNA. DNA polymerase helps in the replication of the “genetic material”. This process initiates with the binding of the enzyme to a promoter sequence in the genetic material (DNA).

b.

Summary Introduction

To determine:

The effect of a mutation in an E. coli gene at the $-35$ locations

Introduction:

Transcription is the basic step of gene expression, in this process, a particular DNA segment with the help of enzyme RNA polymerase forms RNA. DNA polymerase helps in the replication of the “genetic material”. This process initiates with the binding of the enzyme to a promoter sequence in the genetic material (DNA).

c.

Summary Introduction

To determine:

The effect of a mutation in an E. coli gene at the $-20$ locations

Introduction:

Transcription is the basic step of gene expression, in this process, a particular DNA segment with the help of enzyme RNA polymerase forms RNA. DNA polymerase helps in the replication of the “genetic material”. This process initiates with the binding of the enzyme to a promoter sequence in the genetic material (DNA).

d.

Summary Introduction

To determine:

The effect of a mutation in an E. coli gene at the start site locations

Introduction:

Transcription is the basic step of gene expression, in this process, a particular DNA segment with the help of enzyme RNA polymerase forms RNA. DNA polymerase helps in the replication of the “genetic material”. This process initiates with the binding of the enzyme to a promoter sequence in the genetic material (DNA).