Laboratory Experiments in Microbiology (11th Edition)
Laboratory Experiments in Microbiology (11th Edition)
11th Edition
ISBN: 9780321994936
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 14, Problem 3CT

If a bacterium cannot ferment glucose, why not test its ability to ferment other carbohydrates?

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Identify the function of the following enzymes: Desulfurase
If the result of an unknown bacteria is “poor growth” and “red growth,” can this result be interpreted as “organism does not ferment mannitol?” Explain why or why not. If the result of an unknown bacteria is “good growth” and “yellow growth,” is the unknown bacteria more likely Staphylococcus aureus or Staphylococcus epidermidis?
You are given a mixed culture of 3 bacteria with following characteristics- 1) Bacteria 1 - Methicillin- resistant Staphylococcus aureus (MRSA) (does not ferment any of these 4 sugars but forms endospores and is sensitive to penicillin) 2) Bacteria 2 - Escherichia coli (can ferment glucose, maltose, sucrose and lactose) and also produce carbon dioxide gas on fermenting these 3) Bacteria 3 - Enterobacter aerogenes. (can ferment only sucrose and cannot ferment glucose, maltose and lactose. Also on fermenting sucrose it produces hydrogen sulphide gas.) Also they all give similar looking white-colored colonies on an agar plate. Question: Generate separate pure cultures of these 3 bacteria from the mixed culture using the following tools that you learned in the lab. Use any (some or all) of these tools to generate pure cultures as well as confirm them. Any other technique that you use should be one you learned in lab. No need to give detailed info about how to perform the technique but it…
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