If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32. Introduction : Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

Question

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Answer 1

Question

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

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Answer 2

Question

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Expert Solution & Answer

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Answer 3

Question

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Expert Solution & Answer

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Answer 4

Question

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Expert Solution & Answer

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Answer 5

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Answer 6

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

Answer 7

Chapter 25, Problem 5P

(a)

Summary Introduction

To determine: If any of the four nucleotides were omitted from the incubation mixture, will radioactivity be found in the precipitate when extracts of E.coli containing dATP, dTTP, dGTP, dCTP are labeled with αP-32.

Introduction:

Radioactive labeling is used for the analysis and tracing of the various metabolic reactions occurring inside the cell.

Answer 8

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

Answer 9

(b)

Summary Introduction

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

Answer 10

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Answer 11

(c)

Summary Introduction

To determine: If radioactivity will be formed in the precipitate when β,γ-phosphate are used instead of α-phosphate in the deoxyribonucleotides.

Introduction:

Deoxynucleotides consist of deoxyribose sugar, purine or pyrimidine ring and a phosphodiester bond formed between 5'P and 3'OH in the nucleotide strand.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 25 - Prob. 1P Ch. 25 - Prob. 2P Ch. 25 - Prob. 3P Ch. 25 - Prob. 4P Ch. 25 - Prob. 5P Ch. 25 - Prob. 6P Ch. 25 - Prob. 7P Ch. 25 - Prob. 8P Ch. 25 - Prob. 9P Ch. 25 - Prob. 10P

Concept explainers

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To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

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Chapter 25 Solutions

Concept explainers

Videos

To determine: If there will be labeling of 32P if only labeled dTTP is added in the synthesis mixture. Introduction: Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.

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Chapter 25 Solutions

To determine: If there will be labeling of ³²P if only labeled dTTP is added in the synthesis mixture.

Introduction:

Nucleotides present in DNA include thymine, cytosine, adenine and guanine. They form the strands after synthesis of polynucleotide chain.