Chapter 4, Problem 24Q

Summary Introduction

To calculate: The substrate concentration if the V_max is 100µmole/sec, K_M is 1Mm, and rate is 50µmole/sec.

Introduction: Enzymes are the protein molecules that catalyze the rate of a reaction at different substrate concentrations. Initially, the rate of the reaction increases in a linear way at low substrate concentration. With the increase in substrate concentration, the rate of reaction increases, but up to a certain point. At this point, the rate displays saturation, where adding more substrate no longer increases the reaction rate. In a reaction, the substrate molecules interact with the active site of the enzyme to form a new product.

Summary Introduction

To plot: A graph of rate versus substrate concentration for [S] = 0 to 10Mm.

Introduction: The Michaelis-Menten equation is used for enzyme kinetics reaction.

Summary Introduction

To convert: A graph of rate versus substrate concentration for [S] = 0 to 10Mm to a plot of 1/rate versus 1/[S].

Introduction: Enzyme kinetics is defined as the study of enzyme catalyzed chemical reactions. It indicates a rate of reaction as a function of substrate concentration with $V_{\max }$ and $K_{\text{M}}$ of enzyme.

Summary Introduction

To determine: The reason why a graph of rate versus substrate concentration for [S] = 0 to 10Mm is a straight line.

Introduction: Enzymes are biological protein catalysts which alter the speed of the reaction in the biological system. The chemical reactions which are catalyzed by these enzymes are studied under the enzyme kinetics. The reaction rate is measured based on the rate at which the substrate of the enzymes combines with the enzyme and forms the products.