Most polar phase should be identified from mobile phase and stationary phase in reverse phase column chromatography. Concept Introduction : Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions. There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.

Question

PART1: Put True (T) or False (F) for the following sentences: 1. chromatography" derived from two Greek words "Chroma" meaning color and "graphein" meaning to write. The stationary phase may be a solid, or a liquid supported on a solid or gel. 3. The mobile phase may be 2. Solid. 4. Silica gel is the most common stationary phase in adsorption chromatography. 5. Liquid Chromatography abbreviated as (GC ). 6. In Thin Layer Chromatography, the mobile phase is gas. 7. a = KB/KA, where Kg is the distribution constant. 8. k'B and k'A are the retention factors. 9. Rf, is the distance traveled by the compound ONLY. 10. Chromatography is a physical method of separation.

Answer 1

Question

Chapter 6.3, Problem 19E

(a)

Interpretation Introduction

Interpretation:

Most polar phase should be identified from mobile phase and stationary phase in reverse phase column chromatography.

Concept Introduction :

Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.

There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.

(b)

Interpretation Introduction

Interpretation:

Reverse-phase chromatography is more appropriate in separating peptides than normal-phase chromatography should be explained.

Concept Introduction :

Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.

There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.

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PART1: Put True (T) or False (F) for the following sentences: 1. chromatography" derived from two Greek words "Chroma" meaning color and "graphein" meaning to write. The stationary phase may be a solid, or a liquid supported on a solid or gel. 3. The mobile phase may be 2. Solid. 4. Silica gel is the most common stationary phase in adsorption chromatography. 5. Liquid Chromatography abbreviated as (GC ). 6. In Thin Layer Chromatography, the mobile phase is gas. 7. a = KB/KA, where Kg is the distribution constant. 8. k'B and k'A are the retention factors. 9. Rf, is the distance traveled by the compound ONLY. 10. Chromatography is a physical method of separation.