Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 7.2, Problem 1COMQ
A bacterial cell with an F factor conjugates with an
a.
b.
c. one
d. none of the above.
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Students have asked these similar questions
For bacteria that are F+, Hfr, F', and F- answer the following.
a. Describe the state of the F factor.
b. Which of these cells are donors? Which is the recipient?
c. Which of these donors can convert exconjugants to a donor state?
d. Which of these donors can transfer a donor gene to exconjugants?
e. Describe the results of conjugation (i.e., changes in the recipient and the exconjugant) that allow detection of the state of the F factor in a donor strain.
f. Describe a "partial diploid" and how it originates.
Explain the importance of the following features in conjugating donor bacteria:
a. the origin of transfer
b. the conjugation pilus
c. homologous recombination
d. the relaxosome
e. relaxase
f. T strand DNA
g. pillin protein
Referring to conjugation in bacteria: Mark all cell types for which the following statement is true.
Cell is used as a donor cell in conjugation.
Select one or more:
O a. F+
O b. F-
O c. Hfr
O d. F"
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- The following recombinants are recovered when conjugation occurs between an a+d+g+ donor and an a d'g recipient. at dt g+= 84% ad g+= 6% at dgt = 10% ad g+ = less than 1% Which gene is in the middle? a The genes are located too far apart on the bacterial genome to determine which gene is in the middle. d Insufficient data are provided to answer this question.arrow_forwardDefine the following terms:a. transpositionb. transposable elementc. bacterial transformationd. transductione. conjugationarrow_forward. a. You want to perform an interrupted-mating mappingwith an E. coli Hfr strain that is Pyr+, Met+, Xyl+,Tyr+, Arg+, His+, Mal+, and Strs. Describe anappropriate bacterial strain to be used as theother partner in this mating.b. In an Hfr × F− cross, the pyrE gene enters therecipient in 5 minutes, but at this time point thereare no exconjugants that are Met+, Xyl+, Tyr+,Arg+, His+, or Mal+. The mating is now allowed toproceed for 30 minutes and Pyr+ exconjugants areselected. Of the Pyr+ cells, 32% are Met+, 94% areXyl+, 7% are Tyr+, 59% are Arg+, 0% are His+, and71% are Mal+. What can you conclude about theorder of the genes?arrow_forward
- A bacterial transformation is performed with a donor strain that is resistant to four drugs, A, B, C, and D, and a recipient strain that is sensitive to all four drugs. The resulting recipient cell population is divided and plated onmedia containing various combinations of the drugs. The following table shows the results.a. One of the genes is distant from the other three, which appear to be closely linked. Which is the distant gene? b. What is the likely order of the three closely linked genesarrow_forwardIn a gene mapping experiment, DNA from a prototrophic E. coli strain is transformed into an E. coli strain mutant (auxotrophic) for the genes gal, pro, and lac. Some resulting transformants were found to be auxotrophic only for pro, and some only for gal, but no lac- only auxotrophs were observed. Based upon these results, which gene is in the middle? a. gal b. lac c. pro d. cannot be determined c. the genes are not on the same chromosomearrow_forwardAn E. coli Hfr (high frequency recombination) strain is defined as an E. coli strain that Select one: а. contains a conjugative plasmid in its genomic DNA. contains a mobilizable plasmid in cytosol. С. contains a conjugative plasmid in cytosol. d. contains a mobilizable plasmid in its genomic DNA.arrow_forward
- Genetic information can be transferred horizontally from one bacterial cell to another cell of the "same generation" (versus daughter cells), leading to genetic recombination, by all of the following EXCEPT: A. Gene expression (transcription/translation/protein synthesis) B. Transformation C. Conjugation D. Transduction E. None of the other four answers (all are mechanisms of “horizontal transfer” genetic recombination)arrow_forwardDraw a diagram/figure to explain the conjugation process (e.g. use PowerPoint or draw one by hand and include a photo of it). You should include in the diagram the F- recipient, Hfr Donor and the transconjugant/recombinant recipient. Make sure to include the genes encoding for Leucine, Threonine, Thiamine and Streptomycin resistance in your diagram. How does an Hfr strain of coli transfers chromosomal DNA to an F- strain? What determines how much of the chromosomal DNA is transferred?arrow_forwardIf a researcher accidentally transformed the bacterial two hybrid plasmids ( pKT25-X and pUT18-Y) into a normal strain of E coli that produces adenylate cyclase, his/her experiment will: a. show a high lacZ activity in all tubes (control and experiment) because Proteins X and Y do not need to be interacting in order to activate lacZ gene prescription. b. not work because cells lack cAMP. c. need replicates in order to show true interaction between X and Y.arrow_forward
- Which of the following statements about conjugation is false? After a F- cell has conjugated with an F+ cell, both cells would be F+. Both Hfr and F+ bacteria have the ability to produce a sex pilus. In F+ × F- conjugation, none of the bacterial chromosome is transferred; only the F factor is. A Hfr strain usually transfers the entire bacterial chromosome to the recipient cell. F' × F- conjugation can produce a partially diploid bacterial cell.arrow_forwardFirst picture: 1. What must be added in a minimal media for the recombinants to grow? 2. What must be excluded in a minimal media for the non recombinants to be killed? Second picture: 1. How is the recipient cell different at time D than it was at time A? Choice 1:It has a greater number of genes Choice 2: It has a greater mass of DNA. Choice 3:It contains different genes Choice 4: It contains bacteriophage DNA. 2. At which time can the recipient cell first be described as "recombinant"? A,B,C, or D 3. Which of the following processes is responsible for the shape of the curve at time B? Choice 1:transduction Choice 2:another choice Choice 3:entry of Hfr DNA into the recipient cell Choice 4:entry of DNA from the recipient cell into the Hfr cellarrow_forward. In two isolates (one is resistant to ampicillin and theother is sensitive to ampicillin) of a new bacterium,you found that genes encoding ampicillin resistanceare being transferred into the sensitive strain.a. How would you know that gene transfer is takingplace?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license