Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 8, Problem 20PDQ
A phage-infected bacterial culture was subjected to a series of dilutions, and a plaque assay was performed in each case, with the following results. What conclusion can be drawn in the case of each dilution?
| Dilution Factor | Assay Results | |
| (a) | 104 | All bacteria lysed |
| (b) | 106 | 14 plaques |
| (c) | 108 | 0 plaques |
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A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next?
a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates.
b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.
c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.
d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
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A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 8 plaques on the 10-7 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next?
a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.
c.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates.
d.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.
Chapter 8 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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