1/v (sec/μmol) Enzymes A and B catalyze different reactions but use the same reactant molecule as a substrate. The graph below presents the double-reciprocal plots observed when enzyme A and enzyme B are mixed together in a single test tube containing molecule X. X 12° 1> 0.45 0.40 Y 0.35 0.30 Z 0.25 0.20 0.15 0.10 2.05 0.00 -1.20 -1.00 -0.80 -0.60 -0.40 -0.20 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1/[X] (1/μM) A-B What are the Vmax and the Km values for each enzyme under these conditions? A Vmax = 5.9 μmol sec KM = 1.00 μΜ B Vmax = 10. μmol sec KM = 3.0 μΜ

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 6.4P
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1/v (sec/μmol)
Enzymes A and B catalyze different reactions but use the same reactant molecule as a substrate. The graph
below presents the double-reciprocal plots observed when enzyme A and enzyme B are mixed together in a
single test tube containing molecule X.
X
12° 1>
0.45
0.40
Y
0.35
0.30
Z
0.25
0.20
0.15
0.10
2.05
0.00
-1.20 -1.00 -0.80 -0.60 -0.40 -0.20 0.00 0.20 0.40 0.60 0.80 1.00 1.20
1/[X] (1/μM)
A-B
What are the Vmax and the Km values for each enzyme
under these conditions?
A
Vmax
= 5.9
μmol
sec
KM
=
1.00 μΜ
B
Vmax = 10.
μmol
sec
KM = 3.0 μΜ
Transcribed Image Text:1/v (sec/μmol) Enzymes A and B catalyze different reactions but use the same reactant molecule as a substrate. The graph below presents the double-reciprocal plots observed when enzyme A and enzyme B are mixed together in a single test tube containing molecule X. X 12° 1> 0.45 0.40 Y 0.35 0.30 Z 0.25 0.20 0.15 0.10 2.05 0.00 -1.20 -1.00 -0.80 -0.60 -0.40 -0.20 0.00 0.20 0.40 0.60 0.80 1.00 1.20 1/[X] (1/μM) A-B What are the Vmax and the Km values for each enzyme under these conditions? A Vmax = 5.9 μmol sec KM = 1.00 μΜ B Vmax = 10. μmol sec KM = 3.0 μΜ
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