Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis

Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.4: The Hemacytometer

Problem 1CT

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Which of the following tests will help me differentiate between an unknown bacteria that is either Salmonella typhimurium and Serratia marcescens Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Catalase Test
The following is a Mannitol Salt Agar plate. What does the yellow color on the right side of this plate indicate? TAW ASM's MicrobeLibrary Ⓒ Reynolds tolerance of the salt mannitol metabolism tolerance of the dyes and chemicals in the agar lactose metabolism
How can I explain the results of my findings in lamen terms? 1 - Name of Test Type of Medium 2 - Color Before Incubation 3-Color After Incubation? 4 – Results Positive/ Negative? 5 - Reagents Added Lactose Fermentation test Phenol Red Lactose (PRL) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Glucose Fermentation Test Phenol Red Glucose (PRG) Red Color: Yellow Gas Bubble: Yes Positive Phenol Red to detect color change (added) Citrate Utilization Simmons Citrate Green Color: Blue Positive Bromthymol Blue (added) Indole Test Media = SIM Clear Indole: Color: Clear Negative For Indole, we add Kovac’s Hydrogen Sulfide Test Media = SIM clear Hydrogen Sulfide: Color: Clear Negative Iron already added Methyl Red Test (MR test) Media = MRVP Broth Clear Color: Yellow Negative We add Methyl Red Reagent Voges-Proskauer Test (VP test) Media = MRVP Broth Clear…
which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation, catalase test, oxidase test, PCR test, urea hydrolysis test, aglutination test, northern and southern test.) can be used on the disease causing organism below that can help draw a flow chart or ditochomy key? And will the test results in positive or negative to these disease. Folliculitis Schistosomiasis Gas gangrene Infectious mononucleosis Primary Amebic Meningeocephalopathy Traveler's Diarrhea Blastomycosis Hensen's Disease Bacteria Vaginosis Mumps
indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…
Negative staining is useful for OA.motility purposes OB.visualizing morphology OC.flagella detection OD.identifying the capsule
Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…
Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood Agar - 48 hours Growth on Chocolate Agar Results: Growth on Blood Agar 48 hours Growth on Chocolate Agar
Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test
Need help Can a pure culture be prepared from a mixed culture in a broth or plate? Explain.
Picture in white is acid fast other picure is negative stain so far this is the information I have: gram stain: positive motility: motile oxygen requirement: Facultative anaerobe and than I need these two acid fast stain: _____??? negative stain: _____????
which of these tests (i.e gram stain, acid fast stain, endospore, oxidation to fermentation, catalase test, oxidase test, PCR test, urea hydrolysis test, aglutination test, northern and southern test.) can be used on the disease causing organism below that can help draw a flow chart or ditochomy key? And will the test results in positive or negative to these these. -Viral gastroenteritis-Plague-Staphylococcal Scalded Skin Syndrome -Cryptosporidiosis -Peptic Ulcer Disease-Campylobacteria Disease-Beef Tapeworm Infestation-C. Diff Diarrhea-Traveler's Diarrhea