Principles of Biology
2nd Edition
ISBN: 9781259875120
Author: Robert Brooker, Eric P. Widmaier Dr., Linda Graham Dr. Ph.D., Peter Stiling Dr. Ph.D.
Publisher: McGraw-Hill Education
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Question
Chapter 11.6, Problem 1CC
Summary Introduction
To analyze:
The component of the CRISPR Cas system that is associated with the recognition of bacteriophage DNA.
Introduction:
Bacteriophages are the viruses that infect and replicate within bacteria. The structure of bacteriophage includes the protein that encapsulates either DNA or RNA. Some species of bacteria have a CRISPER-Cas system that is effective in defense against the bacteriophages.
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Outline the three stages of CRISPR-Cas action.
Which component of the CRISPR-Cas system directly recognizes thebacteriophage DNA?
In Crispr cas9, is crispr DNA or RNAse? And what is cas9?
Chapter 11 Solutions
Principles of Biology
Ch. 11.1 - Which of these binding interactions might be...Ch. 11.1 - Prob. 1TYKCh. 11.1 - When an ncRNA functions as a decoy, it contains...Ch. 11.2 - Prob. 1BCCh. 11.2 - Prob. 1CCCh. 11.2 - Prob. 1TYKCh. 11.3 - Prob. 1CCCh. 11.3 - Prob. 1TYKCh. 11.4 - Explain why RISC binds to a specific mRNA. What...Ch. 11.4 - The process of RNA interference may lead to the...
Ch. 11.4 - To catalyze the methylation of an rRNA, a snoRNA...Ch. 11.5 - Prob. 1BCCh. 11.5 - Prob. 1TYKCh. 11.6 - Prob. 1CCCh. 11.6 - Which of the following components are needed for...Ch. 11.6 - Prob. 2TYKCh. 11.7 - Prob. 1TYKCh. 11 - Which of the following types of molecules could...Ch. 11 - Prob. 2TYCh. 11 - Prob. 3TYCh. 11 - Prob. 4TYCh. 11 - Prob. 5TYCh. 11 - Prob. 6TYCh. 11 - Prob. 7TYCh. 11 - Prob. 8TYCh. 11 - Prob. 9TYCh. 11 - Prob. 10TYCh. 11 - An ncRNA may have the following funtions:...Ch. 11 - Prob. 2CCQCh. 11 - Prob. 3CCQCh. 11 - Review the concept of an RNA world described in...Ch. 11 - Prob. 2CBQ
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- what are BIOLOGICAL IMPLICATIONS OF CRISPR-CAS9?arrow_forwardDiagram the mechanism by which CRISPR-cas functions in the immune system of bacteria.arrow_forwardNow that you understand how the CRISPR-Cas9 system works, think back to the experiments discussed in the introduction to this chapter, in which researchers used CRISPR-Cas9 genome editing to treat mice with Duchenne muscular dystrophy. Why did the researchers choose to cut out the entire exon 23 in the mice with the disorder? Why not replace the specific mutation using a donor piece of DNA and homologous recombination? Propose some possible explanations.arrow_forward
- a) Previous research had found odd sequences, spacers, and repeats, within some bacterial genomes. Explain what discovery was made to find out what these elements are, one that moved “spacers” from the category of “random and uninteresting” to essential for the CRISPR-Cas9 system. b) What would happen if a bacterial colony was exposed to two phases at once? Is it possible that they survive this exposure? Describe exactly what is expected to see in CRISPR locus if the bacterial cell survived this exposure.arrow_forwardDescribe the process of cloning a DNA fragment into theBamHI and PstI sites of the vector pUC18. How would youscreen for clones that contain an insert? and explain the process(steps) by drawingarrow_forwardExplain How Single-nucleotide mutations can be introduced into the genome using an engineered CRISPR-Cas9 system.arrow_forward
- What role do CRISPR-Cas systems naturally play in bacteria?arrow_forwardWhat is the difference between nonhomologous end-joining (NHEJ) and homology-directed repair (HDR) in the context of genome editing?arrow_forwardWhat evidence demonstrates that CRISPR-Cas is an adaptive immune system defense against viruses?arrow_forward
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